Wienecke R, Zöchling N, Neubert U, Schlüpen E M, Meurer M, Volkenandt M
Department of Dermatology, Ludwig-Maximilians-University of Munich, Germany.
J Invest Dermatol. 1994 Jul;103(1):19-22. doi: 10.1111/1523-1747.ep12388947.
Recently, three subtypes of Borrelia burgdorferi have been identified: Borrelia burgdorferi sensu stricto, Borrelia garinii, and the VS 461 group of Borrelia burgdorferi. These subtypes differ by nucleotide sequence variations within several Borrelia burgdorferi specific genes and most likely by their pathogenetic potential. To assess whether different subtypes of Borrelia burgdorferi might be associated with different cutaneous manifestations and clinical courses of Lyme disease, lesional skin biopsies from 35 patients with erythema migrans and 18 patients with acrodermatitis chronica atrophicans were analyzed. A Borrelia burgdorferi specific gene segment encoding a 26-kD protein with subtype specific nucleotide sequence variations was amplified by a nested polymerase chain reaction technique. For molecular subtyping, the products were transcribed into complementary RNA. Upon polyacrylamide gel electrophoresis, complementary RNA molecules separate into several metastable conformational forms resulting in patterns of bands highly specific for the nucleotide sequence of the transcribed molecules. In biopsy specimens of erythema migrans, the VS 461 subtype was detected in 28 of 35 and the Borrelia garinii subtype in six of 35 cases. In one of 35 cases of erythema migrans Borrelia burgdorferi sensu stricto as well as Borrelia garinii was detected. In contrast, in all 18 biopsies of acrodermatitis chronica atrophicans, only the VS 461 subtype was identified. This subtype is rarely found in the USA, where acrodermatitis chronica atrophicans is almost unknown. These data indicate that acrodermatitis chronica atrophicans might be closely associated with the VS 461 group of Borrelia burgdorferi.
最近，已鉴定出伯氏疏螺旋体的三种亚型：狭义伯氏疏螺旋体、伽氏疏螺旋体和伯氏疏螺旋体VS 461组。这些亚型在几个伯氏疏螺旋体特异性基因内的核苷酸序列变异不同，其致病潜力很可能也不同。为评估伯氏疏螺旋体的不同亚型是否可能与莱姆病的不同皮肤表现和临床病程相关，对35例游走性红斑患者和18例慢性萎缩性肢端皮炎患者的皮损活检标本进行了分析。通过巢式聚合酶链反应技术扩增了编码具有亚型特异性核苷酸序列变异的26-kD蛋白的伯氏疏螺旋体特异性基因片段。为进行分子亚型分型，将产物转录为互补RNA。在聚丙烯酰胺凝胶电泳时，互补RNA分子分离成几种亚稳态构象形式，从而产生对转录分子核苷酸序列具有高度特异性的条带模式。在游走性红斑的活检标本中，35例中有28例检测到VS 461亚型，35例中有6例检测到伽氏疏螺旋体亚型。在35例游走性红斑病例中的1例中，同时检测到狭义伯氏疏螺旋体和伽氏疏螺旋体。相比之下，在所有18例慢性萎缩性肢端皮炎的活检标本中，仅鉴定出VS 461亚型。这种亚型在美国很少见，在那里慢性萎缩性肢端皮炎几乎不为人知。这些数据表明，慢性萎缩性肢端皮炎可能与伯氏疏螺旋体VS 461组密切相关。
