Mani C, Selvakumar N, Narayanan S, Narayanan P R
Tuberculosis Research Centre, Chetput, Chennai 600 031, Tamil Nadu, India.
J Clin Microbiol. 2001 Aug;39(8):2987-90. doi: 10.1128/JCM.39.8.2987-2990.2001.
Mutations in the 81-bp rifampin resistance-determining region (RRDR) of the rpoB gene were analyzed by DNA sequencing of 50 Mycobacterium tuberculosis clinical isolates (44 resistant and 6 sensitive) from various parts of India. Fifty-three mutations of 18 different kinds, 17 point mutations and one deletion, were observed in 43 of 44 resistant isolates. Three novel mutations and three new alleles within the RRDR, along with two novel mutations outside the RRDR, are reported in this study.
通过对来自印度各地的50株结核分枝杆菌临床分离株(44株耐药株和6株敏感株)进行DNA测序,分析了rpoB基因81bp利福平耐药决定区(RRDR)的突变情况。在44株耐药分离株中的43株中观察到18种不同类型的53个突变,其中17个点突变和1个缺失突变。本研究报告了RRDR内的3个新突变和3个新等位基因,以及RRDR外的2个新突变。