通过对栀子提取物的β-葡萄糖苷酶依赖性绿色反应鉴定出致龋放线菌。
Cariogenic actinomyces identified with a beta-glucosidase-dependent green color reaction to Gardenia jasminoides extract.
作者信息
Chen L, Ma L, Park N H, Shi W
机构信息
School of Dentistry and Dental Research Institute, University of California, Los Angeles, California 90095, USA.
出版信息
J Clin Microbiol. 2001 Aug;39(8):3009-12. doi: 10.1128/JCM.39.8.3009-3012.2001.
Abstract
The oral bacteria Actinomyces naeslundii and Actinomyces viscosus are known to contribute to the initiation and progression of human dental caries, especially root caries. We report that both A. naeslundii and A. viscosus react with a component in the Gardenia jasminoides extract to produce a distinct green product. This green color reaction was found to be dependent on the bacterial beta-glucosidase. The reaction is specific for cariogenic actinomyces, and it can detect as few as 10(4) cells of A. naeslundii and A. viscosus per ml.
摘要
已知口腔细菌内氏放线菌和黏性放线菌会导致人类龋齿尤其是根龋的发生和发展。我们报告称，内氏放线菌和黏性放线菌均会与栀子提取物中的一种成分发生反应，生成一种独特的绿色产物。发现这种绿色显色反应依赖于细菌β-葡萄糖苷酶。该反应对致龋放线菌具有特异性，每毫升能检测到低至10⁴个内氏放线菌和黏性放线菌细胞。
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