Tannock G W
Department of Microbiology, University of Otago, PO Box 56, Dunedin, New Zealand.
Curr Issues Mol Biol. 1999;1(1-2):53-64.
Selective culture media and phenotypic tests enable lactobacilli to be differentiated from morphologically similar bacteria. The accurate identification of Lactobacillus species can be accomplished by reference to 16S rRNA gene sequences. Species-specific, PCR primers that target the 16S-23S rRNA spacer region are available for a limited number of Lactobacillus species. Molecular methods for the comprehensive identification of Bifidobacterium species are not yet available. Only DNA-DNA reassociation provides a reliable means of species identification for this genus at present. Bifidobacteria can be differentiated from morphologically similar bacteria by the use of genus-specific, PCR primers or oligonucleotide probes.
选择性培养基和表型试验能够将乳酸杆菌与形态相似的细菌区分开来。通过参考16S rRNA基因序列可实现乳酸杆菌种的准确鉴定。针对有限数量的乳酸杆菌种，有靶向16S - 23S rRNA间隔区的种特异性PCR引物。目前尚无用于双歧杆菌种全面鉴定的分子方法。目前只有DNA - DNA重新结合为该属提供了可靠的种鉴定方法。双歧杆菌可通过使用属特异性PCR引物或寡核苷酸探针与形态相似的细菌区分开来。
