细菌在弗吉尼亚牡蛎（Crassostrea virginica）汞生物累积中的作用。
Role of bacteria in bioaccumulation of mercury in the oyster Crassostrea virginica.
作者信息
Sayler G S, Nelson J D, Colwell R R
出版信息
Appl Microbiol. 1975 Jul;30(1):91-6. doi: 10.1128/am.30.1.91-96.1975.
An investigation of mercury-resistant bacteria was undertaken to determine their role in the accumulation of mercury in a simplified food chain. Oysters (Crassostrea virginica) were maintained in a closed system, sealed aquarium with stirred, aerated water containing 10 mug of 203-HgCl2 per liter. Uptake of 203-Hg by oysters held under control conditions was compared with that of 203-Hg uptake by oysters under similar conditions except that mercury-accumulating and mercury-metabolizing species of Pseudomonas, isolated from Chesapeake Bay, were added to the experimental oysters. After incubation for 4 days, the major portion ofthe 203-Hg in the water column was found to be associated with the microparticulate fraction, corresponding to a rise in total viable count. Mercury accumulation in the oysters was significantly higher in the gill and visceral tissue than other tissue. Mercury concentrations were 200 times greater in tissue fractions of oysters dosed with mercury-metabolizing bacteria compared with the oysters held under control conditions without mercury-metabolizing bacteria.
开展了一项针对耐汞细菌的调查，以确定它们在简化食物链中汞积累过程中的作用。将牡蛎（弗吉尼亚巨蛎）置于一个封闭系统中，即密封的水族箱，箱内装有每升含10微克203 - 氯化汞且经过搅拌、充气的水。将处于对照条件下的牡蛎对203 - 汞的摄取情况，与在类似条件下的牡蛎对203 - 汞的摄取情况进行比较，不同之处在于，从切萨皮克湾分离出的假单胞菌属中能够积累汞和代谢汞的菌株被添加到了实验牡蛎中。孵育4天后，发现水柱中大部分的203 - 汞与微粒部分相关联，这与总活菌数的增加相对应。牡蛎鳃和内脏组织中的汞积累量显著高于其他组织。与未添加汞代谢细菌的对照条件下的牡蛎相比，添加了汞代谢细菌的牡蛎组织部分中的汞浓度高出200倍。
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