Ahn S K, Jiang S J, Hwang S M, Choi E H, Lee J S, Lee S H
Department of Dermatology, Yonsei University Wonju College of Medicine, Korea.
Arch Dermatol Res. 2001 Jun;293(6):308-18. doi: 10.1007/s004030100226.
Acute barrier disruption, regardless of the method of induction, depletes the stratum corneum intercellular lipids and this stimulates a series of lipid/ DNA synthesis activities which lead to barrier recovery. After barrier disruption by tape stripping, occlusion with a water vapor-impermeable membrane inhibits barrier repair. In this study, we investigated the changes in the murine epidermis after barrier perturbation by tape stripping and three different types of surfactants (Emalex NP-12, ENP-12; sodium dodecyl sulfate, SDS; benzalkonium chloride, BKC). To examine the effect of an artificial barrier, we covered the animals with a water vapor-impermeable membrane for 3 days following barrier disruption and then exposed them to the air for 2 days. The histological findings after occlusion or air exposure were similar. However, after air exposure for 2 days, the thickness of the epidermis including the stratum corneum and the stratum granulosum layers decreased to about half that of the epidermis after occlusion. Ultrastructural examination revealed obvious distortion of the lamellar bilayers within the stratum corneum interstices immediately after barrier disruption. After 3 days of occlusion, extensive disorganization was evident in the intercellular domain of the stratum corneum, whereas 2 days after removal of the occlusion, the normal basic unit structure of the lamellar bilayers had partially reappeared. Our findings provide evidence that the kinetic pattern of barrier repair and the morphological changes are similar after occlusion following barrier disruption regardless of the mechanism of disruption.
急性屏障破坏，无论诱导方法如何，都会消耗角质层细胞间脂质，这会刺激一系列脂质/DNA合成活动，从而导致屏障恢复。胶带剥离造成屏障破坏后，用不透水蒸气的膜封闭会抑制屏障修复。在本研究中，我们调查了胶带剥离和三种不同类型的表面活性剂（乳化剂NP - 12、ENP - 12；十二烷基硫酸钠，SDS；苯扎氯铵，BKC）对小鼠表皮屏障扰动后的变化。为了研究人工屏障的作用，我们在屏障破坏后用不透水蒸气的膜覆盖动物3天，然后让它们暴露在空气中2天。封闭或空气暴露后的组织学结果相似。然而，空气暴露2天后，包括角质层和颗粒层在内的表皮厚度降至封闭后表皮厚度的约一半。超微结构检查显示，屏障破坏后立即在角质层间隙内的片层双分子层出现明显扭曲。封闭3天后，角质层细胞间区域出现广泛的紊乱，而去除封闭2天后，片层双分子层的正常基本单位结构部分重新出现。我们的研究结果表明，无论破坏机制如何，屏障破坏后封闭时屏障修复的动力学模式和形态学变化是相似的。
