Ino Julia M, Chevallier Pascale, Letourneur Didier, Mantovani Diego, Le Visage Catherine
Inserm, U698; Cardiovascular Bio-Engineering; X. BichatHospital; Paris, France; Institut Galilée; University Paris 13; Villetaneuse, France.
Laboratory for Biomaterials and Bioengineering; Department of Materials Engineering & University Hospital Research Center; Laval University; Quebec City, QC Canada.
Biomatter. 2013 Oct-Dec;3(4). doi: 10.4161/biom.25414. Epub 2013 Jul 29.
Tailoring the interface interactions between a biomaterial and the surrounding tissue is a capital aspect to consider for the design of medical devices. Poly(vinyl alcohol) (PVA) hydrogels present suitable mechanical properties for various biological substitutes, however the lack of cell adhesion on their surface is often a problem. The common approach is to incorporate biomolecules, either by blending or coupling. But these modifications disrupt PVA intra- and intermolecular interactions leading therefore to a loss of its original mechanical properties. In this work, surface modification by glow discharge plasma, technique known to modify only the surface without altering the bulk properties, has been investigated to promote cell attachment on PVA substrates. N2/H2 microwave plasma treatment has been performed, and the chemical composition of PVA surface has been investigated. X-ray photoelectron and Fourier transform infrared analyses on the plasma-treated films revealed the presence of carbonyl and nitrogen species, including amine and amide groups, while the main structure of PVA was unchanged. Plasma modification induced an increase in the PVA surface wettability with no significant change in surface roughness. In contrast to untreated PVA, plasma-modified films allowed successful culture of mouse fibroblasts and human endothelial cells. These results evidenced that the grafting was stable after rehydration and that it displayed cell adhesive properties. Thus plasma amination of PVA is a promising approach to improve cell behavior on contact with synthetic hydrogels for tissue engineering.
对于医疗设备的设计而言，调整生物材料与周围组织之间的界面相互作用是一个需要考虑的关键方面。聚乙烯醇（PVA）水凝胶具有适用于各种生物替代物的机械性能，然而其表面缺乏细胞粘附性往往是个问题。常见的方法是通过共混或偶联来引入生物分子。但这些修饰会破坏PVA的分子内和分子间相互作用，从而导致其原始机械性能丧失。在这项工作中，研究了通过辉光放电等离子体进行表面改性，该技术已知仅能修饰表面而不改变本体性能，以促进细胞在PVA底物上的附着。进行了N2/H2微波等离子体处理，并研究了PVA表面的化学成分。对等离子体处理过的薄膜进行的X射线光电子能谱和傅里叶变换红外分析表明，存在羰基和含氮物种，包括胺基和酰胺基，而PVA的主要结构未发生变化。等离子体改性导致PVA表面润湿性增加，表面粗糙度无显著变化。与未处理的PVA相比，等离子体改性薄膜能够成功培养小鼠成纤维细胞和人内皮细胞。这些结果证明，接枝在再水化后是稳定的，并且具有细胞粘附特性。因此，PVA的等离子体胺化是一种有前景的方法，可改善与合成水凝胶接触时细胞在组织工程中的行为。
