a Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus) , Pukyong National University , Busan , Republic of Korea.
b Marine-Integrated Bionics Research Center , Pukyong National University , Busan , Republic of Korea.
J Biomater Sci Polym Ed. 2018 May-Jun;29(7-9):972-983. doi: 10.1080/09205063.2017.1374030. Epub 2017 Sep 6.
Phlorotannins (PH) derived from brown algae have been shown to have biological effects. However, the application of PH in biomedical materials has not been investigated. Here, we investigated the effects of PH on normal human dermal fibroblast (NHDF) proliferation and fabricated a composite hydrogel consisting PH and poly (vinyl alcohol) (PVA) (PVA/PH) by a freezing-thawing method for wound healing applications. Cell proliferation was significantly higher in the PH-treated (0.01 and 0.02%) cells than in non-treated cells. Based on the mechanical properties, the PVA/PH hydrogel had a significantly increased swelling ratio and ultimate strain compared to the PVA hydrogel, but the ultimate tensile strength and tensile modulus were decreased. Additionally, cell attachment and proliferation on the composites were evaluated using NHDFs. The results showed that after 1 and 5 days, cell attachment and proliferation were significantly increased on the PVA/PH hydrogel compared with that on the PVA hydrogel. The findings from this study suggest that the PVA/PH hydrogel may be a candidate biomedical material for wound healing applications.
从褐藻中提取的岩藻黄质（PH）已被证明具有生物效应。然而，PH 在生物医学材料中的应用尚未得到研究。在这里，我们研究了 PH 对正常人类真皮成纤维细胞（NHDF）增殖的影响，并通过冻融法制备了由 PH 和聚乙烯醇（PVA）组成的复合水凝胶（PVA/PH），用于伤口愈合应用。与未经处理的细胞相比，在 PH 处理（0.01 和 0.02%）的细胞中，细胞增殖显著更高。基于机械性能，与 PVA 水凝胶相比，PVA/PH 水凝胶的溶胀比和极限应变显著增加，但极限拉伸强度和拉伸模量降低。此外，还使用 NHDF 评估了细胞在复合材料上的附着和增殖。结果表明，与 PVA 水凝胶相比，在第 1 天和第 5 天，细胞在 PVA/PH 水凝胶上的附着和增殖显著增加。这项研究的结果表明，PVA/PH 水凝胶可能是一种用于伤口愈合应用的候选生物医学材料。
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