Viveros N, Arriaga C, Banda V, Ortega-Pierres M G, Yépez-Mulla L
UIMEIP, Centro Médico Nacional Siglo XXI, México, D.F.
Parasite. 2001 Jun;8(2 Suppl):S257-9. doi: 10.1051/parasite/200108s2257.
In this study we compared the sensitivity of molecular, serologic and parasitologic methods for diagnosis of equine trichinellosis in two abattoirs, one rural and one federal inspection type. Diaphragm muscle samples were obtained from 170 slaughter horses and examined by artificial digestion and PCR. Serum samples from these horses were also analyzed by ELISA. No Trichinella muscle larvae were detected by artificial digestion. However, specific antibodies against Trichinella were detected in 17% and 7% of the serum samples examined from the rural and the federal abattoirs respectively. By PCR, 15% and 2% of the samples from these two abattoirs were Trichinella positive.
在本研究中，我们比较了分子、血清学和寄生虫学方法在两个屠宰场（一个为农村型，一个为联邦检查型）诊断马旋毛虫病的敏感性。从170匹屠宰马获取膈肌样本，通过人工消化和聚合酶链反应（PCR）进行检测。这些马的血清样本也通过酶联免疫吸附测定（ELISA）进行分析。人工消化未检测到旋毛虫肌幼虫。然而，分别在来自农村屠宰场和联邦屠宰场检测的血清样本中，17%和7%检测到了抗旋毛虫的特异性抗体。通过PCR检测，这两个屠宰场的样本中分别有15%和2%为旋毛虫阳性。
