Ryabov E V, Fraser G, Mayo M A, Barker H, Taliansky M
Unit of Virology, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5 DA, United Kingdom.
Virology. 2001 Aug 1;286(2):363-72. doi: 10.1006/viro.2001.0982.
Potato leafroll virus (PLRV) was mechanically transmissible when inocula also contained the umbravirus Pea enation mosaic virus-2 (PEMV-2). In plants infected with PLRV and PEMV-2, PLRV accumulated in clusters of mesophyll cells in both inoculated and systemically infected leaves. No transmissions were obtained by coinoculation with Potato virus Y, Potato virus X (PVX), Tobacco mosaic virus, or Cucumber mosaic virus (CMV), although PLRV was transmissible from mixtures with CMV(ORF4) (a recombinant that contained the movement protein (MP) gene of the umbravirus Groundnut rosette virus (GRV) in place of the CMV MP gene). In contrast, neither a recombinant PVX that expressed GRV MP nor a mutant of CMV(ORF4), in which the CMV 2b gene was untranslatable, was able to help PLRV transmission. Possibly both a cell-to-cell movement function and counterdefense mechanisms such as those that block posttranscriptional gene silencing are involved in movement of PLRV within plants and its mechanical transmission between plants.
当接种物中还含有绒毛病毒豌豆耳突花叶病毒2（PEMV-2）时，马铃薯卷叶病毒（PLRV）可通过机械方式传播。在感染了PLRV和PEMV-2的植物中，PLRV在接种叶和系统感染叶的叶肉细胞簇中积累。与马铃薯Y病毒、马铃薯X病毒（PVX）、烟草花叶病毒或黄瓜花叶病毒（CMV）共同接种未获得传播，尽管PLRV可从与CMV（ORF4）（一种重组体，其运动蛋白（MP）基因包含绒毛病毒花生丛簇病毒（GRV）的MP基因，取代了CMV的MP基因）的混合物中传播。相比之下，表达GRV MP的重组PVX或CMV（ORF4）的突变体（其中CMV 2b基因不可翻译）都不能帮助PLRV传播。细胞间运动功能和诸如阻断转录后基因沉默的防御机制可能都参与了PLRV在植物体内的移动及其在植物间的机械传播。
