Amaral A C, Parizotto N A, Salvini T F
Departamento de Fisioterapia, Universidade Federal de São Carlos, São Carlos/SP, Brasil.
Lasers Med Sci. 2001;16(1):44-51. doi: 10.1007/pl00011336.
We evaluated the effect on mice skeletal muscle regeneration of different doses (2.6, 8.4, and 25 J/cm2) of HeNe laser (lambda 632.8 nm; power, 2.6 mW; spot size, 0.007 cm2) applied directly to intact skin of injured muscle. Muscle injury was induced in both right and left Tibialis anterior (TA) muscles by ACL myotoxin (5 mg/kg). Right TA muscles were irradiated daily for 5 days while contralateral muscles received a sham treatment. Only the 2.6 J/cm2 dose resulted in changes such as increased mitochondrial density and muscle fibre in the TA muscles as compared to sham groups (3280 +/- 704 microns 2 versus 2110 +/- 657 microns 2, p = 0.02). We concluded that the HeNe effect on mouse muscle regeneration is dose-specific: only 2.6 J/cm2 increased muscle fibre area and mitochondrial density.
我们评估了将不同剂量（2.6、8.4和25 J/cm²）的氦氖激光（波长632.8 nm；功率2.6 mW；光斑大小0.007 cm²）直接照射到受伤肌肉的完整皮肤上对小鼠骨骼肌再生的影响。通过注射ACL肌毒素（5 mg/kg）在左右两侧胫骨前肌（TA）诱导肌肉损伤。右侧TA肌肉每天照射5天，而对侧肌肉接受假处理。与假处理组相比，只有2.6 J/cm²剂量导致TA肌肉出现线粒体密度增加和肌纤维变化等情况（3280±704平方微米对2110±657平方微米，p = 0.02）。我们得出结论，氦氖激光对小鼠肌肉再生的影响具有剂量特异性：只有2.6 J/cm²增加了肌纤维面积和线粒体密度。
