Imanaka M, Sasaki K, Nemoto S, Ueda E, Murakami E, Miyata D, Tonogai Y
Okayama Prefectural Institute for Environmental Science and Public Health: 739-1, Uchio, Okayama 701-0298, Japan.
Shokuhin Eiseigaku Zasshi. 2001 Apr;42(2):71-8. doi: 10.3358/shokueishi.42.71.
An analytical method using GC/MS was developed for bisphenol A (BPA) in foods and BPA was determined in canned foods and fresh foods such as vegetables, fruit and meat. BPA was extracted with acetone from the samples and the extract was concentrated at under 40 degrees C in vacuo to afford an aqueous solution, which was washed with hexane after alkalization and extracted with 50% diethyl ether-hexane after acidification. Extracts were cleaned up on a PSA and/or a C18 cartridge column, and BPA was derivatized with heptafluorobutyric anhydride and determined by GC/MS (SIM). This method was applicable to the detection and determination of BPA residues in food samples at the level of 1 ng/g. Among canned foods, BPA was found in 6 corned beef, 1 chicken, 9 sweet corn and 3 bean samples at the levels of 17-602 ng/g, 212 ng/g, 2.3-75 ng/g and 3.5-26 ng/g, respectively. BPA was also detected in 1 retort soup and 1 retort pack product at the levels of 11 ng/g and 86 ng/g, respectively. As for dairy products, BPA was not detected in butter and milk. Among fresh foods, BPA was detected in 2 fish and 3 liver samples at the levels of trace (tr)-6.2 ng/g and tr-2.2 ng/g, respectively. In vegetables, fruits and chocolates, a trace level of BPA was detected in only 1 chocolate. Traces of BPA were also detected in 3 samples of 6 boxed lunches.
建立了一种采用气相色谱/质谱联用（GC/MS）测定食品中双酚A（BPA）的分析方法，并测定了罐装食品以及蔬菜、水果和肉类等新鲜食品中的BPA。用丙酮从样品中提取BPA，提取物在40℃以下真空浓缩得到水溶液，碱化后用己烷洗涤，酸化后用50%乙醚-己烷萃取。提取物在PSA和/或C18柱上进行净化，BPA用七氟丁酸酐衍生化后通过GC/MS（选择离子监测模式）测定。该方法适用于食品样品中1 ng/g水平的BPA残留的检测和测定。在罐装食品中，在6份腌牛肉、1份鸡肉、9份甜玉米和3份豆类样品中检测到BPA，含量分别为17 - 602 ng/g、212 ng/g、2.3 - 75 ng/g和3.5 - 26 ng/g。在1份蒸煮汤和1份蒸煮包装产品中也检测到BPA，含量分别为11 ng/g和86 ng/g。至于乳制品，在黄油和牛奶中未检测到BPA。在新鲜食品中，在2份鱼类和3份肝脏样品中检测到BPA，含量分别为痕量（tr）- 6.2 ng/g和tr - 2.2 ng/g。在蔬菜、水果和巧克力中，仅在1份巧克力中检测到痕量的BPA。在6份盒饭中的其中3份中也检测到痕量的BPA。
