Cruz-Filho A M, Sousa-Neto M D, Saquy P C, Pécora J D
Faculty of Dentistry, University of Ribeirão Preto, Brazil.
J Endod. 2001 Mar;27(3):183-4. doi: 10.1097/00004770-200103000-00011.
The effect of EDTAC (ethylenediamine tetra-acetic acid plus Cetavion, an ammonium surfactant), CDTA (cyclohexane-1,2-diaminetetra-acetic acid), and EGTA (ethylene glycol-bis-(beta-amino-ethyl ether) N,N,N',N'-tetra-acetic acid) on the microhardness of radicular dentin of the cervical third of human teeth was studied. Five newly extracted maxillary incisors were sectioned transversely at the cementoenamel junction, and the crowns were discarded. The roots were embedded in blocks of high-speed polymerized acrylic resin and cut transversely into 1-mm sections. The second slice of the cervical third of the root of each tooth was sectioned and divided into four parts. Each part was placed on an acrylic disc that was used as a base for microhardness measurement. Fifty microliters of 15% EDTAC, 1% CDTA, or 1% EGTA were applied to the dentin surface. Deionized and distilled water was used as control. Dentin microhardness was then measured with a Vicker's microhardness apparatus with a load of 50 g for 15 s. Statistical analysis showed that the three chelating solutions significantly reduced dentin microhardness when compared with water; however, there was no statistically significant difference among the three solutions.
研究了乙二胺四乙酸（EDTAC，乙二胺四乙酸加十六烷基三甲基溴化铵，一种铵类表面活性剂）、环己烷-1,2-二胺四乙酸（CDTA）和乙二醇双（β-氨基乙醚）N,N,N',N'-四乙酸（EGTA）对人牙颈部三分之一处牙根牙本质显微硬度的影响。将五颗新拔除的上颌切牙在牙骨质釉质交界处横向切片，去除牙冠。将牙根嵌入高速聚合丙烯酸树脂块中，横向切成1毫米厚的切片。将每颗牙齿牙根颈部三分之一的第二片切片再切成四份。每一份放在用作显微硬度测量基底的丙烯酸圆盘上。将50微升15%的EDTAC、1%的CDTA或1%的EGTA涂覆在牙本质表面。用去离子蒸馏水作为对照。然后用维氏显微硬度仪在50克载荷下加载15秒测量牙本质显微硬度。统计分析表明，与水相比，三种螯合溶液均显著降低了牙本质显微硬度；然而，三种溶液之间没有统计学上的显著差异。
