Lau D T, Shaw P C, Wang J, But P P
Department of Biology, The Chinese University of Hong Kong, Shatin.
Planta Med. 2001 Jul;67(5):456-60. doi: 10.1055/s-2001-15818.
Herba Dendrobii (Shihu) is a commonly used Chinese medicine derived from the stem of several orchid species belonging to the genus Dendrobium. It is rather expensive and adulteration is frequent. Proper authentication of the medicinal species is necessary to protect consumers and support conservation measures. DNA sequences of the internal transcribed spacer 2 (ITS 2) of 16 Dendrobium species were shown to be significantly different from one another by an average of 12.4% and from non-orchids and Pholidota (an adulterant of Shihu) by 29.8% and 18.8%, respectively. The intra-specific variation among the Dendrobium species studied was only about 1%. Therefore, ITS 2 regions could be adopted as a molecular marker for differentiating medicinal Dendrobium species from one another and also from non-orchids and adulterants.
石斛是一种常用的中药材，来源于多种石斛属兰花的茎。它价格昂贵，掺假现象屡见不鲜。对药用物种进行正确鉴定对于保护消费者和支持保护措施至关重要。16种石斛的内部转录间隔区2（ITS 2）的DNA序列显示，它们彼此之间平均有12.4%的显著差异，与非兰花植物和石仙桃（石斛的一种掺假品）分别有29.8%和18.8%的差异。在所研究的石斛物种中，种内变异仅约1%。因此，ITS 2区域可作为一种分子标记，用于区分药用石斛物种之间以及与非兰花植物和掺假品的差异。
