Krawitt E L, Sampson H W, Katagiri C A
Calcif Tissue Res. 1975 Jul 25;18(2):119-24. doi: 10.1007/BF02546232.
Rats raised on laboratory diet and given 20% ethanol in water as a sole source of fluid for 12 days were given 0.2 mug of 1,25-dihydroxycholecalciferol 24 h before measuring duodenal calcium transport by an in vitro everted gut sac technique. This vitamin D metabolite did not reverse the ethanol-induced inhibitiom of transport capacity. Ultrastructural studies of intestinal epithelial cells from animals ingesting ethanol demonstrated changes in mitochondria and other subcellular organelles as well as accumulation of electron-dense, calcium-containing granules in microvilli and a paucity of granules in mitochondria. The results suggest that the inhibition is mediated at the intestinal level, affecting mechanisms which are probably independent of Vitamin D, and in part by an interference in transport of calcium from microvilli to other intracellular sites.
用实验室饲料饲养的大鼠，以含20%乙醇的水溶液作为唯一液体来源，持续12天。在通过体外外翻肠囊技术测量十二指肠钙转运前24小时，给这些大鼠注射0.2微克的1,25 - 二羟基胆钙化醇。这种维生素D代谢产物并未逆转乙醇诱导的转运能力抑制。对摄入乙醇的动物的肠上皮细胞进行超微结构研究发现，线粒体和其他亚细胞器发生了变化，微绒毛中出现电子致密的含钙颗粒积聚，而线粒体中的颗粒则较少。结果表明，这种抑制作用是在肠道水平介导的，影响的机制可能独立于维生素D，部分原因是干扰了钙从微绒毛向其他细胞内位点的转运。
