Basudde C D, Humphreys D J
Calcif Tissue Res. 1975 Jul 25;18(2):133-9. doi: 10.1007/BF02546234.
The oral administration of an aqueous extract of 2.5 g of the dried leaves of Solanum malacoxylon (DLSM) produced a rapid hyperphosphataemia, which becomes apparent 4 to 8 h after treatment, in the rabbit. This effect was not accompanied by any significant change in plasma calcium, mangesium, total protein, albumin, urea and creatinine concentration. The urinary excretion of calcium, magnesium, and inorganic phosphate was markedly increased 24 and 48 h after treatment with the extract and was not accompanied by any significant change in the urinary excretion of hydroxyproline. The hyperphosphataemic effect of the DLSM extract was inhibited by Actinomycin D. It appeared that DLSM does not cause bone resorption in normal rabbits maintained on a diet containing adequate levels of calcium and phosphorus. The increased urinary excretion of calcium magnesium and inorganic phosphate after treatment with DLSM extract appeared to be due to increased intestinal absorption. The absence of any significant change in plasma urea and creatinine concentration after treatment with DLSM extract, and the inhibition of the hyperphosphataemic effect by Actinomycin D, indicated that this was a characteristic response of the rabbit to the active principle, and that it was not due to renal damage. The inhibition of the hyperphosphataemic effect of DLSM by Actinomyein D showed that its action involves the transcription of DNA to RNA and protein synthesis.
给兔子口服2.5克茄属植物软刺茄干燥叶的水提取物（DLSM）后，会迅速出现高磷血症，在治疗后4至8小时变得明显。这种效应并未伴随着血浆钙、镁、总蛋白、白蛋白、尿素和肌酐浓度的任何显著变化。用该提取物治疗24小时和48小时后，尿中钙、镁和无机磷的排泄量显著增加，且羟脯氨酸的尿排泄量没有任何显著变化。DLSM提取物的高磷血症效应被放线菌素D抑制。似乎DLSM在以含有足够钙和磷水平的饮食喂养的正常兔子中不会引起骨吸收。用DLSM提取物治疗后尿中钙、镁和无机磷排泄量增加似乎是由于肠道吸收增加。用DLSM提取物治疗后血浆尿素和肌酐浓度没有任何显著变化，以及放线菌素D对高磷血症效应的抑制，表明这是兔子对活性成分的特征性反应，而不是由于肾损伤。放线菌素D对DLSM高磷血症效应的抑制表明其作用涉及DNA转录为RNA和蛋白质合成。
