Korkmaz A, Oter S, Deveci S, Goksoy C, Bilgic H
Departments of Physiology and Pathology, Gülhane Military Medical Academy, Etlik, Ankara, Turkey.
J Urol. 2001 Sep;166(3):1119-23.
Hyperbaric oxygen therapy and mesna have been successfully used for hemorrhagic cystitis. We defined the protective effects of hyperbaric oxygen and mesna in further cyclophosphamide induced hemorrhagic cystitis in guinea pigs.
A total of 48 male guinea pigs were divided into 6 groups. All groups received 2 doses of 68.1 mg./kg. cyclophosphamide intraperitoneally at the same time intervals but group 1 served as controls. Group 2 received cyclophosphamide only, group 3 received hyperbaric oxygen treatment (2.8 ATA for 90 minutes twice daily) before and the day after further cyclophosphamide, group 4 received 21.5 mg./kg. mesna intraperitoneally only with further cyclophosphamide, group 5 received hyperbaric oxygen and mesna with further cyclophosphamide, and group 6 received hyperbaric oxygen before initial cyclophosphamide, between the 2 doses and after the further dose of cyclophosphamide, and mesna on the days of cyclophosphamide.
Although mesna alone provided protection against cyclophosphamide induced cystitis in animal bladders, there was also significant damage compared with controls. When the uroprotective efficacy of mesna was supported with hyperbaric oxygen, bladder protection was promoted since mean histological scores and hematuria levels in this group did not differ from those in controls.
According to this animal study using hyperbaric oxygen as adjuvant therapy in humans may be a better tool than mesna alone for the prophylaxis and treatment of cyclophosphamide induced hemorrhagic cystitis.
高压氧疗法和美司钠已成功用于治疗出血性膀胱炎。我们进一步确定了高压氧和美司钠对环磷酰胺诱导的豚鼠出血性膀胱炎的保护作用。
总共48只雄性豚鼠被分为6组。所有组均以相同的时间间隔腹腔注射2剂68.1毫克/千克的环磷酰胺，但第1组作为对照组。第2组仅接受环磷酰胺，第3组在进一步注射环磷酰胺之前和之后一天接受高压氧治疗（2.8个绝对大气压，每天两次，每次90分钟），第4组仅在进一步注射环磷酰胺时腹腔注射21.5毫克/千克的美司钠，第5组在进一步注射环磷酰胺时同时接受高压氧和美司钠，第6组在初次注射环磷酰胺之前、两次注射之间以及进一步注射环磷酰胺之后接受高压氧，并且在注射环磷酰胺的日子里接受美司钠。
尽管单独使用美司钠可对动物膀胱中环磷酰胺诱导的膀胱炎起到保护作用，但与对照组相比仍有明显损伤。当美司钠的尿路保护功效得到高压氧的支持时，膀胱保护作用得到增强，因为该组的平均组织学评分和血尿水平与对照组无差异。
根据这项动物研究，在人类中使用高压氧作为辅助治疗可能比单独使用美司钠是预防和治疗环磷酰胺诱导的出血性膀胱炎的更好工具。
