Etlik O, Tomur A, Deveci S, Pişkin I, Pekcan M
Department of Physiology, Gülhane Military Medical Academy, Ankara, Turkey.
J Urol. 1997 Dec;158(6):2296-9. doi: 10.1016/s0022-5347(01)68238-7.
The aim of this research was to compare the protective effects of mesna, hyperbaric oxygenation (HBO), and their combination in cyclophosphamide-induced hemorrhagic cystitis in guinea pigs. Following one dose of i.p. 21.5 mg./kg. mesna administration 20 minutes before i.p. 68.1 mg./kg. cyclophosphamide, 3 additional doses of mesna were given every three hours. A total of 8 HBO exposures, 5 of which were applied prophylactically before cyclophosphamide, were performed at 2.8 ATA for 90 minutes 2 times a day. Although mesna or HBO provided significant protection for cyclophosphamide-cystitis in animal bladders, there was also significant damage compared with controls. The combination of mesna and HBO, which act through independent mechanisms, resulted in complete protection, since mean histological scores and hematuria levels in this group were not different from controls (p >0.05). Therefore, this combination may be a useful tool in the prophylaxis and treatment of cyclophosphamide-induced hemorrhagic cystitis.
本研究的目的是比较美司钠、高压氧（HBO）及其联合应用对环磷酰胺诱导的豚鼠出血性膀胱炎的保护作用。腹腔注射68.1mg/kg环磷酰胺前20分钟腹腔注射一剂21.5mg/kg美司钠，之后每三小时再给予三剂美司钠。每天两次在2.8ATA下进行共8次高压氧暴露，其中5次在环磷酰胺给药前预防性应用，每次90分钟。尽管美司钠或高压氧对动物膀胱中环磷酰胺诱导的膀胱炎有显著保护作用，但与对照组相比仍有明显损伤。美司钠和高压氧通过独立机制发挥作用，二者联合应用产生了完全保护作用，因为该组的平均组织学评分和血尿水平与对照组无差异（p>0.05）。因此，这种联合应用可能是预防和治疗环磷酰胺诱导的出血性膀胱炎的一种有用方法。
