Rahmah N, Lim B H, Khairul Anuar A, Shenoy R K, Kumaraswami V, Lokman Hakim S, Chotechuang P, Kanjanopas K, Ramachandran C P
Department of Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Trans R Soc Trop Med Hyg. 2001 May-Jun;95(3):280-4. doi: 10.1016/s0035-9203(01)90234-2.
An IgG4 ELISA based on a novel recombinant antigen was evaluated for detection of Brugia malayi infection, using 2487 sera from various institutions: 2031 samples from Universiti Sains Malaysia, 276 blinded sera from 2 other institutions in Malaysia, 140 blinded sera from India and 40 blinded sera from Thailand. These sera were from various groups of individuals, i.e., microfilaraemics, chronic patients, endemic normals, non-endemic normals and individuals with other parasitic and bacterial infections. Based on a cut-off optical density reading of 0.300, the IgG4 ELISA demonstrated specificity rates of 95.6-100%, sensitivity rates of 96-100%, positive predictive values of 75-100% and negative predictive values of 98.9-100%. These evaluation studies demonstrated the high specificity and sensitivity of this test for the detection of active B. malayi infection. Thus, the IgG4 ELISA would be very useful as a tool in diagnosis and in elimination programmes for brugian filariasis.
基于一种新型重组抗原的IgG4酶联免疫吸附测定法（ELISA）用于检测马来布鲁线虫感染，该研究使用了来自不同机构的2487份血清：2031份来自马来西亚理科大学，276份来自马来西亚其他2个机构的盲法血清，140份来自印度的盲法血清以及40份来自泰国的盲法血清。这些血清来自不同个体群体，即微丝蚴血症患者、慢性患者、流行区正常人、非流行区正常人以及患有其他寄生虫和细菌感染的个体。基于0.300的临界光密度读数，IgG4 ELISA显示特异性率为95.6 - 100%，灵敏度率为96 - 100%，阳性预测值为75 - 100%，阴性预测值为98.9 - 100%。这些评估研究证明了该检测方法在检测活动性马来布鲁线虫感染方面具有高特异性和灵敏度。因此，IgG4 ELISA作为一种工具在马来丝虫病的诊断和消除计划中将非常有用。
