Mrochek J E, Dinsmore S R, Waalkes T P
Clin Chem. 1975 Aug;21(9):1314-22.
We describe a sensitive, reproducible procedure of analysis for the six neutral carbohydrates in glycoproteins, by high-resolution anion-exchange chromatography. As many as 16 neutral carbohydrates can be separated by elution with a concentration gradient of boric acid (pH 7, 67 to 672 mmol/liter). The carbohydrates are detected with a cerate oxidimetric detector system, which monitors the fluorescence of Ce3+ produced by the reaction of the eluted constituents with Ce4+. Sensitivity to 1 nmol of fucose is demonstrated. Analytical methods and results are presented for mannose, fucose, and galactose in serum glycoproteins for both normal women and those with metastatic breast cancer. We briefly discuss the possibility of separating and analyzing for the three neutral carbohydrates in serum glycoproteins in 4 h by isocratic (constant eluent concentration) elution from a chromatographic column.
我们描述了一种通过高分辨率阴离子交换色谱法分析糖蛋白中六种中性碳水化合物的灵敏且可重复的方法。通过用硼酸浓度梯度（pH 7，67至672 mmol/升）洗脱，多达16种中性碳水化合物可被分离。碳水化合物用铈酸盐氧化imetric检测系统检测，该系统监测洗脱成分与Ce4+反应产生的Ce3+的荧光。证明了对1 nmol岩藻糖的灵敏度。给出了正常女性和转移性乳腺癌女性血清糖蛋白中甘露糖、岩藻糖和半乳糖的分析方法及结果。我们简要讨论了通过从色谱柱等度（恒定洗脱液浓度）洗脱在4小时内分离和分析血清糖蛋白中三种中性碳水化合物的可能性。
