Garcic A, Kratochvíla J
Clin Chim Acta. 1975 Jul 9;62(1):29-34. doi: 10.1016/0009-8981(75)90276-4.
A micromethod is described for the spectrophotometric determination of inorganic phosphate in serum and urine utilizing Rhodamin B as a dye (phosphomolybdat-Rhodamin B complex), Brij 35, and polyvinylpyrrolidon as catalyst. The procedure does not involve deproteinization and yields a stable complex in 20 min. The linearity is constant up to at least 9 mg Pi per 100 ml. It is both accurate (r = 0.972 in serum, r = 0.989 in urine, recovery in urine 98%) and precise (vk = 1.16% in the series). Bilirubin up to 10 mg/100 ml and serum protein do not interfere with the method.
描述了一种微量方法，用于分光光度法测定血清和尿液中的无机磷酸盐，该方法利用罗丹明B作为染料（磷钼酸 - 罗丹明B复合物）、Brij 35和聚乙烯吡咯烷酮作为催化剂。该程序不涉及去蛋白过程，并且在20分钟内产生稳定的复合物。线性在每100 ml至少9 mg磷的范围内保持恒定。该方法既准确（血清中r = 0.972，尿液中r = 0.989，尿液回收率98%）又精确（该系列中变异系数vk = 1.16%）。高达10 mg/100 ml的胆红素和血清蛋白不干扰该方法。
