Kyaw A, Maung-U K, Toe T
Anal Biochem. 1985 Mar;145(2):230-4. doi: 10.1016/0003-2697(85)90354-9.
A new colorimetric procedure is described for inorganic phosphate determination using the color reaction between inorganic phosphate and acidified ammonium molybdate in the presence of Triton X-100. The method is simple and specific, and produces results comparable with those of the widely used method of Fiske-Subbarow [C. H. Fiske and Y. Subbarow (1925) J. Biol. Chem. 66, 375]. The linearity of the standard curve is observed up to an absorbance of 0.410, compared to 0.370 in the Fiske-Subbarow method. Trichloroacetic acid and tungstic acid are found to interfere in the assay. However, the method is not disturbed by nonionic detergents and can therefore be used for the determination of inorganic phosphate contaminated with nonionic detergents.
本文描述了一种新的比色法，用于在Triton X - 100存在下，利用无机磷酸盐与酸化钼酸铵之间的显色反应来测定无机磷酸盐。该方法简单且具有特异性，其结果与广泛使用的Fiske - Subbarow法[C. H. Fiske和Y. Subbarow (1925) J. Biol. Chem. 66, 375]相当。标准曲线在吸光度达到0.410时仍保持线性，而Fiske - Subbarow法为0.370。发现三氯乙酸和钨酸会干扰该测定。然而，该方法不受非离子洗涤剂的干扰，因此可用于测定被非离子洗涤剂污染的无机磷酸盐。
