Wada A M, Reese D E, Bader D M
Stahlman Cardiovascular Laboratories, Program for Developmental Biology and The Division of Cardiovascular Medicine, Vanderbilt University, Nashville, TN, USA.
Development. 2001 Jun;128(11):2085-93. doi: 10.1242/dev.128.11.2085.
Bves is a protein expressed in cells of the developing coronary vascular system, specifically in the proepicardium, migrating epithelial epicardium, delaminated vasculogenic mesenchyme and vascular smooth muscle cells. Here, we show that Bves protein undergoes a dynamic subcellular redistribution during coronary vessel development. Bves is a membrane protein with three predicted transmembrane helices, an extracellular C terminus and an intracellular N terminus, and is confined to the lateral membrane compartment of epithelial cells. When epicardial cells are dissociated into single cells in vitro, Bves accumulates in a perinuclear region until cells make contact, at which time Bves is trafficked to the cell membrane. Bves accumulates at points of cell/cell contact, such as filopodia and cell borders, before the appearance of E-cadherin, suggesting an early role in cell adhesion. While Bves shares no homology with any known adhesion molecule, transfection of Bves into L-cells readily confers adhesive behavior to these cells. Finally, Bves antibodies inhibit epithelial migration of vasculogenic cells from the proepicardium. This study provides direct evidence that Bves is a novel cell adhesion molecule and suggests a role for Bves in coronary vasculogenesis.
Bves是一种在发育中的冠状血管系统细胞中表达的蛋白质，具体表达于前心外膜、迁移的上皮心外膜、分层的血管生成间充质和血管平滑肌细胞中。在此，我们表明Bves蛋白在冠状动脉发育过程中经历动态的亚细胞重新分布。Bves是一种膜蛋白，具有三个预测的跨膜螺旋、一个细胞外C末端和一个细胞内N末端，并局限于上皮细胞的侧膜区室。当体外将心外膜细胞解离为单细胞时，Bves积聚在核周区域，直到细胞接触，此时Bves被转运到细胞膜。在E-钙黏蛋白出现之前，Bves积聚在细胞/细胞接触点，如丝状伪足和细胞边界，表明其在细胞黏附中起早期作用。虽然Bves与任何已知的黏附分子均无同源性，但将Bves转染到L细胞中可使这些细胞轻易获得黏附行为。最后，Bves抗体抑制来自前心外膜的血管生成细胞的上皮迁移。本研究提供了直接证据表明Bves是一种新型细胞黏附分子，并提示Bves在冠状动脉血管生成中发挥作用。
