PACAP 1-38 as neurotransmitter in the porcine antrum.

作者信息

Tornøe K, Hannibal J, Georg B, Schmidt P T, Hilsted L, Fahrenkrug J, Holst J J

机构信息

Department of Medical Physiology, The Panum Institute, University of Copenhagen, Blegdamsvej 3, Dk-2200, N, Copenhagen, Denmark.

出版信息

Regul Pept. 2001 Sep 15;101(1-3):109-21. doi: 10.1016/s0167-0115(01)00276-2.

UNLABELLED

The concentration of PACAP 1-38 in porcine antrum amounted to 15.4+/-7.9 and 20.3+/-8 pmol/g tissue in the mucosal and muscular layers. PACAP immunoreactive (IR) fibres innervated the muscular (co-localised with VIP) and submucosal/mucosal layers (some co-storing VIP and CGRP) including myenteric and submucosal plexus and blood vessels. Only myenteric nerve cell bodies contained PACAP-IR (co-storing VIP). In isolated perfused antrum, vagus nerve stimulation (8 Hz) and capsaicin (10(-5) M) increased PACAP 1-38 release. PACAP 1-38 (10(-9) M) increased substance P (SP), gastrin releasing peptide (GRP) and VIP release. PACAP 1-38 (10(-8) M) inhibited gastrin secretion and stimulated somatostatin secretion and motility dose-dependently. PACAP-induced motility was strongly inhibited by the antagonist PACAP 6-38 but also by atropine and substance P-antagonists (CP99994/SR48968) but PACAP 6-38 had no effect on vagus-induced secretion or motility.

CONCLUSION

PACAP 1-38 may be involved in antral motility and secretion by interacting with cholinergic, SP-ergic, GRP-ergic and/or VIP-ergic neurones, and may also be involved in afferent reflex pathways.