Czegledi Levente, Csosz Eva, Gulyas Gabriella
Department of Animal Science, Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen, 138 Boszormenyi Street, 4032 Debrecen, Hungary.
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, 98 Nagyerdei Square, 4032 Debrecen, Hungary.
Animals (Basel). 2022 Jun 16;12(12):1559. doi: 10.3390/ani12121559.
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic and multifunctional neuropeptide; it takes part in the regulation of various physiological processes, such as feeding, reproduction, catecholamine synthesis, thermoregulation, motor activity, brain development and neuronal survival. Since PACAP plays important regulatory roles, we hypothesized that the level of PACAP in blood is associated with expression of other proteins, which are involved in different metabolic pathways. The objective of the present study was to compare plasma protein profiles of cows with high and low plasma PACAP levels. Differential proteome analyses were performed by two-dimensional gel electrophoresis (2D-PAGE) followed by tryptic digestion and protein identification by liquid chromatography−mass spectrometry (LC-MS). A total of 210 protein spots were detected, and 16 protein spots showed statistically significant differences (p < 0.05) in the expression levels between groups. Ten spots showed a higher intensity in the high-PACAP-concentration group, while six spots were more abundant in the low-PACAP-concentration group. The functions of the differentially expressed proteins indicate that the PACAP level of plasma is related to the lipid metabolism and immune status of cattle.
垂体腺苷酸环化酶激活多肽（PACAP）是一种具有多效性和多功能的神经肽；它参与多种生理过程的调节，如进食、繁殖、儿茶酚胺合成、体温调节、运动活动、大脑发育和神经元存活。由于PACAP发挥着重要的调节作用，我们推测血液中PACAP的水平与参与不同代谢途径的其他蛋白质的表达有关。本研究的目的是比较血浆PACAP水平高和低的奶牛的血浆蛋白质谱。通过二维凝胶电泳（2D-PAGE）进行差异蛋白质组分析，随后进行胰蛋白酶消化，并通过液相色谱-质谱联用（LC-MS）进行蛋白质鉴定。共检测到210个蛋白质斑点，其中16个蛋白质斑点在两组之间的表达水平上显示出统计学上的显著差异（p < 0.05）。10个斑点在高PACAP浓度组中强度较高，而6个斑点在低PACAP浓度组中更为丰富。差异表达蛋白质的功能表明，血浆PACAP水平与牛的脂质代谢和免疫状态有关。
