冷冻保存的人类精子的顶体蛋白酶活性。

Acrosin activity of cryo-preserved human spermatozoa.

作者信息

Schill W B

出版信息

Fertil Steril. 1975 Jul;26(7):711-20.

Abstract

The acrosin activity of human spermatozoa was determined in fresh, glycerolated, and cryo-preserved semen specimens. In 119 semen specimens, mean increases in acrosin activity of 62% in glycerolated and 56% in cryo-preserved samples were found. Thus, no statistically significant differences in mean acrosin activity were found between glycerolated and cryo-preserved spermatozoa. However, 23.5% of the cryo-preserved samples showed a significant decrease in extractable acrosin activity when compared with untreated controls. In oligospermic specimens (less than 40 million spermatozoa/ml), a statistically significant decrease in acrosin activity due to cryo-injury was detectable. Differentiation of specimens responding to glycerol pretreatment with an increase in extractable acrosin activity from those responding with a decrease showed a different freezing behavior, indicating two ejaculate types with respect to acrosin extraction. Samples frozen without glycerol protection showed the same amounts of extractable acrosin activity as did glycerol-protected specimens. The stability of acrosin in acidic acrosomal extracts during liquid nitrogen freeze treatment was confirmed.

摘要

在新鲜、甘油化和冷冻保存的精液标本中测定了人类精子的顶体蛋白酶活性。在119份精液标本中，发现甘油化样本的顶体蛋白酶活性平均增加62%，冷冻保存样本的顶体蛋白酶活性平均增加56%。因此，甘油化精子和冷冻保存精子的平均顶体蛋白酶活性在统计学上没有显著差异。然而，与未处理的对照相比，23.5%的冷冻保存样本显示可提取的顶体蛋白酶活性显著降低。在少精子症标本（精子浓度低于4000万/ml）中，可检测到由于冷冻损伤导致的顶体蛋白酶活性在统计学上显著降低。对甘油预处理有反应且可提取的顶体蛋白酶活性增加的标本与反应性降低的标本之间的差异表明了不同的冷冻行为，这表明在顶体蛋白酶提取方面存在两种射精类型。未用甘油保护冷冻的样本显示出与甘油保护标本相同量的可提取顶体蛋白酶活性。证实了顶体蛋白酶在酸性顶体提取物中液氮冷冻处理期间的稳定性。