Henkel R, Menkveld R, Kleinhappl M, Schill W B
Centre for Dermatology and Andrology, Justus Liebig University, Gaffkystr. 14, D-35385 Giessen, Germany.
J Assist Reprod Genet. 2001 Jul;18(7):371-7. doi: 10.1023/a:1016618405570.
The aim of this study was to investigate possible seasonal changes in human sperm parameters, especially chromatin condensation.
In a first run, 3155 patients attending the andrological outpatient clinic at the Centre of Dermatology and Andrology at Justus Liebig University, Giessen, Germany, from January 1992 to October 1995 were examined for sperm count, motility, vitality, and chromatin condensation.
The respective results were correlated according to season. Significant seasonal changes were observed in chromatin condensation and sperm count, with mean maximum values (for chromatin condensation and sperm count) of 86.24% aniline blue-negative spermatozoa in January and 68.75 x 10(6) mL-1 in April. To confirm the observation of seasonal changes in sperm chromatin condensation in Germany on the Southern Hemisphere, 179 patients attending the Reproductive Biology Unit at Tygerberg Hospital, Tygerberg, South Africa, were examined by means of the aniline blue stain from April 1999 to April 2000. For chromatin condensation, a significant seasonal change shifted by 4-5 months was observed on the Southern Hemisphere. However, no seasonal variations could be found for the sperm count.
Our results clearly demonstrate seasonal changes in sperm count and chromatin condensation. In contrast, no circannual relation was observed for motility and vitality.
本研究旨在调查人类精子参数可能存在的季节性变化，尤其是染色质凝聚情况。
在第一轮研究中，对1992年1月至1995年10月期间在德国吉森尤斯图斯·利比希大学皮肤病与男科学中心男科门诊就诊的3155名患者进行了精子计数、活力、存活率及染色质凝聚情况的检测。
将各项结果按季节进行相关性分析。观察到染色质凝聚和精子计数存在显著的季节性变化，1月份苯胺蓝阴性精子的平均最大值（染色质凝聚和精子计数方面）为86.24%，4月份精子计数为68.75×10⁶/mL⁻¹。为了证实德国精子染色质凝聚的季节性变化在南半球也存在，1999年4月至2000年4月期间，对南非泰格堡泰格堡医院生殖生物学科的179名患者采用苯胺蓝染色法进行了检测。在南半球观察到染色质凝聚的显著季节性变化出现了4至5个月的偏移。然而，精子计数未发现季节性变化。
我们的结果清楚地表明精子计数和染色质凝聚存在季节性变化。相比之下，未观察到活力和存活率与年度周期的关系。
