Coghlan L, Utzinger U, Richards-Kortum R, Brookner C, Zuluaga A, Gimenez-Conti I, Follen M
Department of Veterinary Sciences, The University of Texas M.D. Anderson Cancer Center, Science Park, Bastrop, Texas, USA.
Lasers Surg Med. 2001;29(1):1-10. doi: 10.1002/lsm.1078.
The hamster cheek pouch carcinogenesis model, using chronic treatments of dimethylbenz[alpha]anthracene (DMBA) was used as a model system to investigate changes in epithelial tissue autofluorescence throughout the dysplasia-carcinoma sequence.
STUDY DESIGN/MATERIALS AND METHODS: Fluorescence emission spectra were measured weekly from 42 DMBA-treated animals and 20 control animals at 337, 380, and 460 nm excitation. A subset of data in which histopathology was available was used to develop diagnostic algorithms to separate neoplastic and non-neoplastic tissue. The change in fluorescence intensity over time was examined in all samples at excitation-emission wavelength pairs identified as diagnostically useful.
Algorithms based on autofluorescence can separate neoplastic and non-neoplastic tissue with 95% sensitivity and 93% specificity. Greatest contributions to diagnostic algorithms are obtained at 380 nm excitation, and 430, 470, and 600 nm emission. Changes in fluorescence intensity are apparent as early as 3 weeks after initial treatment with DMBA, whereas morphologic changes associated with dysplasia occur on average at 7.5-12.5 weeks after initial treatment.
Fluorescence spectroscopy provides a potential tool to identify biochemical changes associated with dysplasia and hyperplasia, which precede morphologic changes observed in histologically stained sections.
采用二甲基苯并[a]蒽（DMBA）长期处理的仓鼠颊囊癌发生模型作为一种模型系统，用于研究上皮组织自体荧光在发育异常-癌序列中的变化。
研究设计/材料与方法：每周对42只经DMBA处理的动物和20只对照动物在337、380和460nm激发波长下测量荧光发射光谱。利用一组有组织病理学数据的子集来开发诊断算法，以区分肿瘤组织和非肿瘤组织。在被确定为具有诊断价值的激发-发射波长对下，对所有样本中荧光强度随时间的变化进行了检查。
基于自体荧光的算法能够以95%的灵敏度和93%的特异性区分肿瘤组织和非肿瘤组织。在380nm激发波长以及430、470和600nm发射波长下，对诊断算法的贡献最大。在用DMBA初次处理后3周，荧光强度的变化就很明显，而与发育异常相关的形态学变化平均在初次处理后7.5 - 12.5周出现。
荧光光谱法提供了一种潜在工具，可用于识别与发育异常和增生相关的生化变化，这些变化先于组织学染色切片中观察到的形态学变化。
