一种用于皮肤恶性肿瘤光谱诊断的临床仪器的设计与验证
Design and validation of a clinical instrument for spectral diagnosis of cutaneous malignancy.
作者信息
Rajaram Narasimhan, Aramil Timothy J, Lee Kelvin, Reichenberg Jason S, Nguyen Tri H, Tunnell James W
机构信息
Department of Biomedical Engineering, The University of Texas at Austin, 1 University Station C0800, Austin, Texas 78712, USA.
出版信息
Appl Opt. 2010 Jan 10;49(2):142-52. doi: 10.1364/AO.49.000142.
Abstract
We report a probe-based portable and clinically compatible instrument for the spectral diagnosis of melanoma and nonmelanoma skin cancers. The instrument combines two modalities--diffuse reflectance and intrinsic fluorescence spectroscopy--to provide complementary information regarding tissue morphology, function, and biochemical composition. The instrument provides a good signal-to-noise ratio for the collected reflectance and laser-induced fluorescence spectra. Validation experiments on tissue phantoms over a physiologically relevant range of albedos (0.35-0.99) demonstrate an accuracy of close to 10% in determining scattering, absorption and fluorescence characteristics. We also demonstrate the ability of our instrument to collect in vivo diffuse reflectance and fluorescence measurements from clinically normal skin, dysplastic nevus, and malignant nonmelanoma skin cancer.
摘要
我们报告了一种基于探头的便携式且临床兼容的仪器，用于黑色素瘤和非黑色素瘤皮肤癌的光谱诊断。该仪器结合了两种模式——漫反射和固有荧光光谱——以提供有关组织形态、功能和生化组成的补充信息。该仪器为收集的反射率和激光诱导荧光光谱提供了良好的信噪比。在生理相关反照率范围（0.35 - 0.99）内对组织模型进行的验证实验表明，在确定散射、吸收和荧光特性方面的准确度接近10%。我们还展示了我们的仪器从临床正常皮肤、发育异常痣和恶性非黑色素瘤皮肤癌中收集体内漫反射和荧光测量值的能力。
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