Liu Y, Huang H, Chen Y
Department of Pathology, Third Military Medical University, Chongqing 400038.
Zhonghua Zheng Xing Shao Shang Wai Ke Za Zhi. 1999 Nov;15(6):419-23.
To investigate the role and significance of PAF in the early pulmonary injury following burns combined with endotoxemia in rats.
An animal model, in which rats were subjected to 20% TBSA III degree burns combined with intraperitoneal injection of lipopolysaccharide (LPS), was used for this experiment. The changes in the pulmonary morphology were observed by LM, EM and immunohistochemistry(IHC). The contents of PAF and TNF-alpha in the plasma were determined with quantitative analysis. PAF receptor antagonist(BN50739) was used to treat early pulmonary injury.
PAF was one of the inflammatory mediators to precede TNF changes during burns combined with endotoxemia in the early stage of pulmonary injury. The contents of PAF in the plasma was positively correlated to the extent of pulmonary injury, which may be alleviated after injection of PAF receptor antagonist (BN50739).
The results suggest that PAF plays an very important pathogenic role in the early lung injury resulted from burns combined with endotoxemia.
探讨血小板活化因子（PAF）在大鼠烧伤合并内毒素血症后早期肺损伤中的作用及意义。
采用大鼠20%体表面积Ⅲ度烧伤并腹腔注射脂多糖（LPS）的动物模型进行本实验。通过光镜、电镜及免疫组化观察肺组织形态学变化。采用定量分析法测定血浆中PAF和肿瘤坏死因子-α（TNF-α）的含量。应用PAF受体拮抗剂（BN50739）治疗早期肺损伤。
在烧伤合并内毒素血症肺损伤早期，PAF是先于TNF变化的炎症介质之一。血浆中PAF含量与肺损伤程度呈正相关，注射PAF受体拮抗剂（BN50739）后肺损伤可减轻。
结果表明PAF在烧伤合并内毒素血症所致早期肺损伤中起非常重要的致病作用。
