Wang X, Liu Y, Feng J
Department of Pathology, 3rd Military Medical University, Chongqing 400038.
Zhonghua Zheng Xing Shao Shang Wai Ke Za Zhi. 1999 Mar;15(2):95-8.
To observe the distribution and cell localization of TNF-alpha mRNA and its protein and study their role on pathogenesis of liver injury in rats.
An animal model in which rats were subjected to 20% TBSA III degree burns combined with intraperitoneal injection of lipopolysaccharide (LPS) was used for this experiment. The changes in the hepatic morphology, functions and TNF-alpha content in the serum and the expression, localization of liver TNF-alpha and TNF-alpha mRNA were determined with LM and EM, quantitative analysis, immunohistochemistry(IHC) and in situ hybridization (ISH).
It was showed that there were sinusoid reaction, KCs activation and degeneration, necrosis of HCs, and platelets aggregation, fibrin deposition and PMNs attachment in sinusoid. The activity of ALT was obviously elevated and the content of ALB was slightly lowered. The contents of TNF-alpha in serum showed the peak values at 3 h. TNF-alpha was mainly localized in sinusoid endothelial cells(SECs), Kupffer cells (KCs) and TNF-alpha mRNA was mainly distributed in KCs, polymorphonuclear neutrophils (PMNs) and macrophages(MPs).
The results suggest that the expression and localization of TNF-alpha mRNA and its protein are in agreement with the pathological changes of liver injury. TNF-alpha is one of the key cytokines in pathogenesis of liver injury in rats after burns complicated by endotoxemia.
观察肿瘤坏死因子-α（TNF-α）mRNA及其蛋白在大鼠体内的分布和细胞定位，并研究它们在大鼠肝损伤发病机制中的作用。
本实验采用大鼠20%体表面积Ⅲ度烧伤并腹腔注射脂多糖（LPS）的动物模型。运用光镜（LM）、电镜（EM）、定量分析、免疫组织化学（IHC）和原位杂交（ISH）技术，检测肝脏形态、功能变化，血清中TNF-α含量，以及肝脏TNF-α和TNF-α mRNA的表达与定位。
结果显示，肝血窦出现反应，枯否细胞（KCs）活化、变性，肝细胞（HCs）坏死，血窦内有血小板聚集、纤维蛋白沉积和中性粒细胞（PMNs）黏附。谷丙转氨酶（ALT）活性明显升高，白蛋白（ALB）含量略有降低。血清中TNF-α含量在3小时达到峰值。TNF-α主要定位于肝血窦内皮细胞（SECs）、枯否细胞（KCs），而TNF-α mRNA主要分布于KCs、多形核中性粒细胞（PMNs）和巨噬细胞（MPs）。
结果表明，TNF-α mRNA及其蛋白的表达和定位与肝损伤的病理变化一致。TNF-α是烧伤合并内毒素血症大鼠肝损伤发病机制中的关键细胞因子之一。
