Wang Xiaodong, Liu Yousheng, Feng Junming
Department of Pathology, Third Military Medical University Chongqing 400038, Ch ina.
Chin J Traumatol. 1999 May 15;2(1):30-34.
To observe tissue distribution and cell localization of TNF-alpha mRNA and its protein and study their role in the pathogenesis of liver injury in burn rats. METHODS: An animal model of rats subjected to 20% TBSA III degree burns combined with intraperitoneal injection of lipopolysaccharide (LPS) was used for this experiment. The changes of hepatic morphology and functions and serum TNF-alpha content and expression and localization of liver TNF-alpha and TNF-alpha mRNA were determined with light microscope (LM) and electron microscope (EM), quantitative analysis, immunohistochemistry (IHC) and in situ hybridization (ISH). RESULTS: It showed that there were sinusoid reaction, KCs activation and degeneration, necrosis of HCs, and platelets aggregation, fibrins deposition and PMNs attachment in sinusoid. The activity of ALT was obviously elevated and ALB content was slightly decreased. The serum content of TNF-alpha showed peak at 3 hours. TNF-alpha was mainly localized in sinusoid endothelial cells (SECs) and Kupffer cells (KCs), and TNF-alpha mRNA was mainly distributed in KCs, polymorphonuclears neutrophils (PMNs) and macrophages (MPs). CONCLUSIONS: It suggests that TNF-alpha mRNA and its protein expression and localization are coincident with the pathological changes of liver injury. TNF-alpha is one of the key cytokines in the pathogenesis of liver injury in burn rats with endotoxemia.
观察TNF-α mRNA及其蛋白的组织分布和细胞定位，探讨其在烧伤大鼠肝损伤发病机制中的作用。方法：采用20% 体表面积Ⅲ度烧伤合并腹腔注射脂多糖（LPS）的大鼠动物模型进行实验。应用光镜（LM）、电镜（EM）、定量分析、免疫组织化学（IHC）和原位杂交（ISH）技术，检测肝组织形态和功能的变化、血清TNF-α含量以及肝组织中TNF-α和TNF-α mRNA的表达与定位。结果：结果显示，肝血窦出现反应、库普弗细胞（KCs）活化及变性、肝细胞（HCs）坏死，血窦内有血小板聚集、纤维蛋白沉积及中性粒细胞（PMNs）黏附。谷丙转氨酶（ALT）活性明显升高，白蛋白（ALB）含量略有下降。血清TNF-α含量在伤后3小时达到峰值。TNF-α主要定位于肝血窦内皮细胞（SECs）和库普弗细胞（KCs），TNF-α mRNA主要分布于KCs、多形核中性粒细胞（PMNs）和巨噬细胞（MPs）。结论：提示TNF-α mRNA及其蛋白的表达与定位和肝损伤的病理变化一致。TNF-α是内毒素血症烧伤大鼠肝损伤发病机制中的关键细胞因子之一。
