Baay M F, Tjalma W A, Weyler J, Goovaerts G, Buytaert P, Van Marck E A, Lardon F, Vermorken J B
Dept of Medical Oncology, University of Antwerp, Belgium.
Eur J Gynaecol Oncol. 2001;22(3):204-8.
Worldwide there is a strong relation between the presence of human papillomavirus (HPV) and the development of cervical cancer. This study investigated the prevalence and genotype of HPV in women with normal smears, women with premalignant lesions and women with cervical cancer in Antwerp, Belgium. Type-specific polymerase chain reaction (PCR) for HPV types 16 and 18 and general primer PCR (GP5+/6+) was performed on DNA extracted from paraffin-embedded tissue from women with lesions or fresh material from controls. HPV was detected in 11% of controls, 61% of women with atypia, 77% of women with CIN lesions and 88% of women with cervical carcinoma (chi2 trend, 273, p<0.001). The odds ratio for high-risk HPV types was 9.3 for atypia (95%CI. 4.3-19.8), 33.6 for CIN lesions (95%CI, 19.3-58.6) and 78.8 for cervical cancer (95%CI, 39.2-158.3). In total, 19 different HPV genotypes were detected, including five low risk HPV types. Seven of the 14 high-risk HPV types were detected in cervical cancer patients. Based on our study it is suggested that a prophylactic vaccine based on a cocktail of a limited number of high-risk HPV types should be considered in order to protect most women from developing cervical cancer.
在全球范围内，人乳头瘤病毒（HPV）的存在与宫颈癌的发生之间存在着密切关系。本研究调查了比利时安特卫普市宫颈涂片正常的女性、癌前病变女性和宫颈癌女性中HPV的流行情况及基因型。对从有病变女性的石蜡包埋组织或对照组新鲜材料中提取的DNA进行了针对HPV 16型和18型的型特异性聚合酶链反应（PCR）以及通用引物PCR（GP5+/6+）。在11%的对照组、61%的非典型增生女性、77%的宫颈上皮内瘤变（CIN）女性和88%的宫颈癌女性中检测到了HPV（卡方趋势，273，p<0.001）。非典型增生的高危HPV型别的优势比为9.3（95%可信区间，4.3 - 19.8），CIN病变为33.6（95%可信区间，19.3 - 58.6），宫颈癌为78.8（95%可信区间，39.2 - 158.3）。总共检测到19种不同的HPV基因型，包括5种低危HPV型别。在宫颈癌患者中检测到了14种高危HPV型别中的7种。基于我们的研究，建议考虑使用基于有限数量高危HPV型别的混合疫苗进行预防接种，以保护大多数女性预防宫颈癌的发生。
