Nyiredy S
Research Institute for Medicinal Plants, Budakalász, Hungary.
J AOAC Int. 2001 Jul-Aug;84(4):1219-31.
This paper summarizes the role of planar chromatography (PC) in medicinal and aromatic plant (MAP) research and development, and demonstrates the importance of the technique, after extraction, in the analysis of MAP (identification and quantitative determination of the separated compound/s), in the purification and isolation process, and in different types of screening procedure. Special attention is paid to analytical, micropreparative and preparative forced-flow techniques, for example overpressured-layer chromatography (OPLC) and rotation planar chromatography (RPC). The special features of analytical, micropreparative, and preparative layer chromatography (PLC), OPLC, and RPC are compared in tables. Purification and isolation procedures using forced-flow techniques are shown in flowcharts. Some applications, relating to different classes of substance, are presented to demonstrate the versatility of various planar chromatographic techniques.
本文总结了平面色谱法（PC）在药用和芳香植物（MAP）研发中的作用，并证明了该技术在MAP提取后的分析（分离化合物的鉴定和定量测定）、纯化和分离过程以及不同类型筛选程序中的重要性。特别关注了分析、微量制备和制备强制流动技术，例如超压薄层色谱法（OPLC）和旋转平面色谱法（RPC）。表格中比较了分析、微量制备和制备薄层色谱法（PLC）、OPLC和RPC的特点。使用强制流动技术的纯化和分离程序以流程图展示。列举了一些与不同物质类别相关的应用，以证明各种平面色谱技术的多功能性。
