Nyiredy Sz
Research Institute for Medicinal Plants, P.O. Box 11, H-2011 Budakalász, Hungary.
J Chromatogr A. 2003 Jun 6;1000(1-2):985-99. doi: 10.1016/s0021-9673(03)00308-x.
This paper summarizes progress in forced-flow planar chromatography (FFPC) and demonstrates the importance of the different techniques like rotation planar chromatography (RPC), overpressured-layer chromatography (OPLC), and electroplanar chromatography (EPC). Special attention is paid to a novel analytical FFPC method in which continuous development and continuous evaporation of the mobile phase from the end of the chromatographic plate ensure forced-flow development. A simple, but powerful preparative forced-flow technique is also reported; in this technique hydrostatic pressure is used to increase mobile-phase velocity. Parallel- and serially coupled layers open up new vistas for the analysis of a large number of samples (up to 216) for high throughput screening and for the analysis of very complex matrices. The special features of fully off-line and fully on-line RPC, OPLC, and EPC are compared in a table. New detection methods--on-line coupling of OPLC with radiodetection and on-line OPLC-MS--are also discussed. The role of a new spraying device for post-chromatographic chemical detection and for biological detection is also discussed. Some applications, relating to different classes of substances, are given to demonstrate the versatility of the various FFPC techniques.
本文总结了强制流动平面色谱法（FFPC）的进展，并论证了旋转平面色谱法（RPC）、超压层色谱法（OPLC）和电平面色谱法（EPC）等不同技术的重要性。特别关注了一种新型分析FFPC方法，其中从色谱板末端对流动相进行连续展开和连续蒸发可确保强制流动展开。还报道了一种简单但强大的制备性强制流动技术；在该技术中，利用静水压力提高流动相速度。平行和串联耦合层为大量样品（多达216个）的高通量筛选分析以及非常复杂基质的分析开辟了新的前景。以表格形式比较了完全离线和完全在线RPC、OPLC和EPC的特点。还讨论了新的检测方法——OPLC与放射性检测的在线联用以及在线OPLC-MS。还讨论了一种用于色谱后化学检测和生物检测的新型喷雾装置的作用。给出了一些涉及不同类别物质的应用实例，以展示各种FFPC技术的多功能性。
