Plant Protection Institute, Hungarian Academy of Sciences, Herman O. Str. 15, POB 102, Budapest H-1525, Hungary.
J Chromatogr A. 2012 Apr 6;1232:3-18. doi: 10.1016/j.chroma.2011.11.049. Epub 2011 Dec 1.
The pressurized ultramicro (UM) chamber as a closed adsorbent layer chamber enables the use of a special chromatoplate and a pump to increase and optimize the mobile phase flow velocity through an optional development distance in an adsorbent layer. This chamber is the basic instrument of overpressured-layer chromatography (OPLC), which is a separation technique that combines the advantages of conventional TLC/HPTLC with those of HPLC. The versions of OPLC instrument, the character and achievement of off-line and on-line OPLC systems in analytical and preparative use are described. The development of BioArena as a complex bioautographic system means an exploitation of the unique advantages of planar-layer system for detection, isolation and identification of new antimicrobials, antineoplastics, biopesticides and other biologically active substances as well as for studying fundamental biochemical reactions and mechanisms.
加压超微（UM）腔作为封闭的吸附层腔，允许使用特殊的色谱板和泵来增加和优化通过吸附层的可选展开距离的流动相速度。该腔室是过压层色谱（OPLC）的基本仪器，它是一种分离技术，结合了常规 TLC/HPTLC 的优点和 HPLC 的优点。描述了 OPLC 仪器的版本、离线和在线 OPLC 系统在分析和制备用途中的特点和成就。BioArena 作为一个复杂的生物自显影系统的发展意味着利用平面层系统的独特优势，用于检测、分离和鉴定新的抗生素、抗肿瘤药、生物农药和其他生物活性物质，以及研究基本的生化反应和机制。
