Savale H S, Pandya K K, Gandhi T P, Modi I A, Modi R I, Satia M C
Cadila Pharmaceuticals Ltd, R&D Unit, Dholka, Ahmedabad, India.
J AOAC Int. 2001 Jul-Aug;84(4):1252-7.
A rapid and sensitive high performance, thin-layer chromatographic (HPTLC) method has been developed for the measurement of celiprolol in human plasma and its use in pharmacokinetic studies has been evaluated. Detection and quantitation were performed without using an internal standard. A simple extraction procedure was followed for extracting celiprolol from plasma and a known amount of the extract was spotted on precoated silica gel 60 F254 plates using a Camag Linomat IV autosampler. Celiprolol was quantitated using a Camag TLC Scanner 3. The average recovery of authentic analytes (20 to 200 ng/mL) added to plasma was 72.06 +/- 2.8% and the lowest amount of celiprolol that could be detected was 10 ng/mL. The method provides a direct estimate of the amount of celiprolol present in plasma. Pharmacokinetic parameters of 2 marketed preparations have also been determined after oral administration to 12 healthy human volunteers.
已开发出一种快速、灵敏的高效薄层色谱（HPTLC）方法用于测定人血浆中的塞利洛尔，并评估了其在药代动力学研究中的应用。检测和定量无需使用内标。采用简单的萃取程序从血浆中提取塞利洛尔，使用Camag Linomat IV自动进样器将已知量的提取物点样于预涂硅胶60 F254板上。使用Camag TLC Scanner 3对塞利洛尔进行定量。添加到血浆中的真实分析物（20至200 ng/mL）的平均回收率为72.06±2.8%，可检测到的塞利洛尔的最低量为10 ng/mL。该方法可直接估算血浆中塞利洛尔的含量。在对12名健康人类志愿者口服给药后，还测定了2种市售制剂的药代动力学参数。
