Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, 35516, Egypt.
J Fluoresc. 2012 Jul;22(4):1141-50. doi: 10.1007/s10895-012-1053-1. Epub 2012 Apr 3.
Two sensitive fluorometric methods were developed for the determination of both bopindolol malonate (BOP) and celiprolol HCl (CLP) based on measuring their native fluorescence in methanol and acetonitrile, respectively. For BOP, the fluorescence was measured at 316 nm after excitation at 278 nm. The proposed method was successfully applied to the assay of commercial tablets as well as content uniformity testing. For CLP, the fluorescence was enhanced by the addition of carboxymethylcellulose solution and measured at 455 nm after excitation at 339 nm. The method was successfully applied to the analysis of CLP in tablets and biological fluids. In both methods, interference likely to be introduced from co-formulated, co-administered, or chemically related drugs was studied. The results were statistically compared with those obtained by reference methods and were found to be in good agreement.
建立了两种灵敏的荧光法，分别基于在甲醇和乙腈中测量其本征荧光，用于测定丙戊酰心安马来酸盐（BOP）和塞利洛尔盐酸盐（CLP）。对于 BOP，在 278nm 激发下，于 316nm 处测量荧光。该方法成功应用于商业片剂的测定和含量均匀度检验。对于 CLP，通过加入羧甲基纤维素溶液增强荧光，并在 339nm 激发下于 455nm 处测量荧光。该方法成功应用于片剂和生物体液中 CLP 的分析。在这两种方法中，研究了可能来自同配方、同给药途径或化学相关药物的干扰。结果与参考方法得到的结果进行了统计学比较，发现两者吻合良好。
