Abdul Samad S, Yusoff H, Fadilah S A
Department Medical Microbiology and Immunology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur.
Med J Malaysia. 2001 Mar;56(1):32-8.
A biotin-avidin-linked immunosorbent assay was developed to detect Aspergillus antigens in sera of immunocompromised patients. The assay was based on a double antibody sandwich ELISA using polyclonal antibodies raised against water-soluble antigens of Aspergillus fumigatus. Aspergillus antigens were positive in sera of 9 of 16 (56%) patients who were studied prospectively and in 13 of 73 (19%) patients studied retrospectively. The 9 prospectively studied patients who were antigen positive were febrile neutropenic hematological malignancy patients who exhibited a high risk of acquiring invasive aspergillosis.
开发了一种生物素-抗生物素蛋白连接免疫吸附测定法，用于检测免疫功能低下患者血清中的曲霉抗原。该测定法基于双抗体夹心ELISA，使用针对烟曲霉水溶性抗原产生的多克隆抗体。在16例前瞻性研究的患者中，有9例（56%）血清曲霉抗原呈阳性；在73例回顾性研究的患者中，有13例（19%）呈阳性。9例前瞻性研究中抗原呈阳性的患者为发热性中性粒细胞减少的血液系统恶性肿瘤患者，他们发生侵袭性曲霉病的风险很高。
