Wilson E V, Hearn V M, Mackenzie D W
Mycology Reference Laboratory, Central Public Health Laboratory, London, U.K.
J Med Vet Mycol. 1987 Dec;25(6):365-75.
An ELISA for the detection and measurement of Aspergillus antigenaemia has been developed and evaluated by examining sera submitted over a 12-month period from immunocompromised patients with a likelihood of invasive aspergillosis. Results from proven cases of invasive aspergillosis confirmed at post-mortem and specimens from individuals with suspected disease showed that tests on single serum samples were often negative. Multiple specimens from the same patient greatly increased the frequency of detection. Repeated monitoring of sera from a single patient showed wide fluctuations in antigen level, which was considered to be due partly to the medical regimen to which the patient was subject. Control sera from healthy laboratory personnel were consistently negative, but a number of 'at-risk' patients without other evidence of invasive aspergillosis sometimes had low amounts of antigen. Concentrations of Aspergillus antigen of 100 ng ml-1 or higher were considered to be strongly suggestive of fungal invasion.
一种用于检测和测量曲霉抗原血症的酶联免疫吸附测定（ELISA）已被开发出来，并通过检测12个月期间免疫功能低下且有侵袭性曲霉病可能的患者提交的血清进行了评估。尸检确诊的侵袭性曲霉病病例结果以及疑似疾病个体的标本显示，对单份血清样本进行的检测往往呈阴性。来自同一患者的多个标本大大提高了检测频率。对单个患者的血清进行重复监测显示抗原水平波动很大，这部分被认为是由于患者所接受的医疗方案所致。健康实验室工作人员的对照血清始终呈阴性，但一些没有其他侵袭性曲霉病证据的“高危”患者有时抗原量较低。曲霉抗原浓度达到100 ng/ml或更高被认为强烈提示有真菌侵袭。
