与1型人类免疫缺陷病毒跨膜蛋白（gp41）中七肽重复基序对应的肽段可引发针对包膜糖蛋白受体激活构象的抗体。
Peptides corresponding to the heptad repeat motifs in the transmembrane protein (gp41) of human immunodeficiency virus type 1 elicit antibodies to receptor-activated conformations of the envelope glycoprotein.
作者信息
de Rosny E, Vassell R, Wingfield P T, Wild C T, Weiss C D
机构信息
Office of Vaccines, Center for Biologics Evaluation and Research (CBER), Food and Drug Administration (FDA), Bethesda, Maryland 20892-4555, USA.
出版信息
J Virol. 2001 Sep;75(18):8859-63. doi: 10.1128/jvi.75.18.8859-8863.2001.
Abstract
Two heptad repeat regions in the ectodomain of the human immunodeficiency virus type 1 (HIV-1) transmembrane subunit (gp41) self-assemble into a six-helix bundle structure that is critical for virus entry. Immunizations with peptides corresponding to these regions generated antibodies specific to the receptor-activated conformations of gp41.
摘要
人类免疫缺陷病毒1型（HIV-1）跨膜亚基（gp41）胞外域中的两个七肽重复区域自组装成六螺旋束结构，这对病毒进入至关重要。用对应于这些区域的肽进行免疫可产生针对gp41受体激活构象的特异性抗体。
相似文献
1
Peptides corresponding to the heptad repeat motifs in the transmembrane protein (gp41) of human immunodeficiency virus type 1 elicit antibodies to receptor-activated conformations of the envelope glycoprotein.与1型人类免疫缺陷病毒跨膜蛋白（gp41）中七肽重复基序对应的肽段可引发针对包膜糖蛋白受体激活构象的抗体。
J Virol. 2001 Sep;75(18):8859-63. doi: 10.1128/jvi.75.18.8859-8863.2001.
2
Recognition by human monoclonal antibodies of free and complexed peptides representing the prefusogenic and fusogenic forms of human immunodeficiency virus type 1 gp41.人单克隆抗体对代表人类免疫缺陷病毒1型gp41融合前和融合形式的游离及复合肽段的识别。
J Virol. 2000 Jul;74(13):6186-92. doi: 10.1128/jvi.74.13.6186-6192.2000.
3
Oligomer-specific conformations of the human immunodeficiency virus (HIV-1) gp41 envelope glycoprotein ectodomain recognized by human monoclonal antibodies.人源单克隆抗体识别的人类免疫缺陷病毒（HIV-1）gp41包膜糖蛋白胞外域的寡聚体特异性构象。
AIDS Res Hum Retroviruses. 2009 Mar;25(3):319-28. doi: 10.1089/aid.2008.0213.
4
Dissection of human immunodeficiency virus type 1 entry with neutralizing antibodies to gp41 fusion intermediates.利用针对gp41融合中间体的中和抗体剖析1型人类免疫缺陷病毒的进入过程。
J Virol. 2002 Jul;76(13):6780-90. doi: 10.1128/jvi.76.13.6780-6790.2002.
5
Binding of HIV-1 gp41-directed neutralizing and non-neutralizing fragment antibody binding domain (Fab) and single chain variable fragment (ScFv) antibodies to the ectodomain of gp41 in the pre-hairpin and six-helix bundle conformations.HIV-1 gp41导向的中和及非中和性片段抗体结合域（Fab）和单链可变片段（ScFv）抗体与处于前发夹构象和六螺旋束构象的gp41胞外域的结合。
PLoS One. 2014 Aug 8;9(8):e104683. doi: 10.1371/journal.pone.0104683. eCollection 2014.
6
A conformation-specific monoclonal antibody reacting with fusion-active gp41 from the human immunodeficiency virus type 1 envelope glycoprotein.一种与来自人类免疫缺陷病毒1型包膜糖蛋白的融合活性gp41发生反应的构象特异性单克隆抗体。
J Virol. 1998 Dec;72(12):10213-7. doi: 10.1128/JVI.72.12.10213-10217.1998.
7
Antigenic properties of the human immunodeficiency virus transmembrane glycoprotein during cell-cell fusion.细胞间融合过程中人类免疫缺陷病毒跨膜糖蛋白的抗原特性
J Virol. 2002 Dec;76(23):12123-34. doi: 10.1128/jvi.76.23.12123-12134.2002.
8
Six-helix bundle completion in the distal C-terminal heptad repeat region of gp41 is required for efficient human immunodeficiency virus type 1 infection.六螺旋束的形成是 gp41 远端 C 端七肽重复区发挥功能所必需的，这对于高效的人类免疫缺陷病毒 1 型感染是必需的。
Retrovirology. 2018 Apr 2;15(1):27. doi: 10.1186/s12977-018-0410-9.
9
Truncation of the cytoplasmic domain induces exposure of conserved regions in the ectodomain of human immunodeficiency virus type 1 envelope protein.人免疫缺陷病毒1型包膜蛋白胞质结构域的截短会诱导其胞外结构域中保守区域的暴露。
J Virol. 2002 Mar;76(6):2683-91. doi: 10.1128/jvi.76.6.2683-2691.2002.
10
Epitope map of human immunodeficiency virus type 1 gp41 derived from 47 monoclonal antibodies produced by immunization with oligomeric envelope protein.源自用寡聚包膜蛋白免疫产生的47种单克隆抗体的1型人类免疫缺陷病毒gp41的表位图谱。
J Virol. 1997 Apr;71(4):2674-84. doi: 10.1128/JVI.71.4.2674-2684.1997.
引用本文的文献
1
Utilizing Machine Learning to Improve Neutralization Potency of an HIV-1 Antibody Targeting the gp41 N-Heptad Repeat.利用机器学习提高靶向gp41 N-七肽重复序列的HIV-1抗体的中和效力。
ACS Chem Biol. 2025 Jul 18;20(7):1470-1480. doi: 10.1021/acschembio.5c00035. Epub 2025 Jun 20.
2
A Derivative of the D5 Monoclonal Antibody That Targets the gp41 N-Heptad Repeat of HIV-1 with Broad Tier-2-Neutralizing Activity.一种针对 HIV-1 gp41 N-七肽重复序列的 D5 单克隆抗体衍生物，具有广泛的 Tier-2 中和活性。
J Virol. 2021 Jul 12;95(15):e0235020. doi: 10.1128/JVI.02350-20.
3
The high-affinity immunoglobulin receptor FcγRI potentiates HIV-1 neutralization via antibodies against the gp41 N-heptad repeat.高亲和力免疫球蛋白受体 FcγRI 通过针对 gp41 N-七肽重复序列的抗体增强 HIV-1 中和作用。
Proc Natl Acad Sci U S A. 2021 Jan 19;118(3). doi: 10.1073/pnas.2018027118.
4
HIV-1 gp41 Residues Modulate CD4-Induced Conformational Changes in the Envelope Glycoprotein and Evolution of a Relaxed Conformation of gp120.HIV-1 gp41 残基调节包膜糖蛋白中 CD4 诱导的构象变化和 gp120 构象的弛豫演化。
J Virol. 2018 Jul 31;92(16). doi: 10.1128/JVI.00583-18. Print 2018 Aug 15.
5
Nonneutralizing Antibodies Induced by the HIV-1 gp41 NHR Domain Gain Neutralizing Activity in the Presence of the HIV Fusion Inhibitor Enfuvirtide: a Potential Therapeutic Vaccine Strategy.由HIV-1 gp41 NHR结构域诱导产生的非中和抗体在HIV融合抑制剂恩夫韦肽存在的情况下获得中和活性：一种潜在的治疗性疫苗策略。
J Virol. 2015 Jul;89(13):6960-4. doi: 10.1128/JVI.00791-15. Epub 2015 Apr 22.
6
Elevated temperature triggers human respiratory syncytial virus F protein six-helix bundle formation.高温引发人类呼吸道合胞病毒 F 蛋白六螺旋束的形成。
Virology. 2010 Jan 20;396(2):226-37. doi: 10.1016/j.virol.2009.10.040. Epub 2009 Nov 18.
7
ADS-J1 inhibits human immunodeficiency virus type 1 entry by interacting with the gp41 pocket region and blocking fusion-active gp41 core formation.ADS-J1 通过与 gp41 口袋区域相互作用并阻断融合活性 gp41 核心形成来抑制人类免疫缺陷病毒 1 型进入。
Antimicrob Agents Chemother. 2009 Dec;53(12):4987-98. doi: 10.1128/AAC.00670-09. Epub 2009 Sep 28.
8
Conformational changes in HIV-1 gp41 in the course of HIV-1 envelope glycoprotein-mediated fusion and inactivation.HIV-1包膜糖蛋白介导的融合和失活过程中HIV-1 gp41的构象变化。
Biochemistry. 2005 Sep 20;44(37):12471-9. doi: 10.1021/bi051092d.
9
The C terminus of the B5 receptor for herpes simplex virus contains a functional region important for infection.单纯疱疹病毒B5受体的C末端包含一个对感染很重要的功能区域。
J Virol. 2005 Jun;79(12):7431-7. doi: 10.1128/JVI.79.12.7431-7437.2005.
10
Binding of the 2F5 monoclonal antibody to native and fusion-intermediate forms of human immunodeficiency virus type 1 gp41: implications for fusion-inducing conformational changes.2F5单克隆抗体与人类免疫缺陷病毒1型gp41天然形式及融合中间形式的结合：对融合诱导构象变化的影响
J Virol. 2004 Mar;78(5):2627-31. doi: 10.1128/jvi.78.5.2627-2631.2004.
本文引用的文献
1
Evidence that the transition of HIV-1 gp41 into a six-helix bundle, not the bundle configuration, induces membrane fusion.有证据表明，是HIV-1 gp41转变为六螺旋束，而非六螺旋束的结构，引发了膜融合。
J Cell Biol. 2000 Oct 16;151(2):413-23. doi: 10.1083/jcb.151.2.413.
2
Recognition by human monoclonal antibodies of free and complexed peptides representing the prefusogenic and fusogenic forms of human immunodeficiency virus type 1 gp41.人单克隆抗体对代表人类免疫缺陷病毒1型gp41融合前和融合形式的游离及复合肽段的识别。
J Virol. 2000 Jul;74(13):6186-92. doi: 10.1128/jvi.74.13.6186-6192.2000.
3
A conformation-specific monoclonal antibody reacting with fusion-active gp41 from the human immunodeficiency virus type 1 envelope glycoprotein.一种与来自人类免疫缺陷病毒1型包膜糖蛋白的融合活性gp41发生反应的构象特异性单克隆抗体。
J Virol. 1998 Dec;72(12):10213-7. doi: 10.1128/JVI.72.12.10213-10217.1998.
4
Mutational analysis of residues in the coiled-coil domain of human immunodeficiency virus type 1 transmembrane protein gp41.人类免疫缺陷病毒1型跨膜蛋白gp41卷曲螺旋结构域中残基的突变分析。
J Virol. 1998 Dec;72(12):9676-82. doi: 10.1128/JVI.72.12.9676-9682.1998.
5
HIV entry and its inhibition.HIV病毒的侵入及其抑制
Cell. 1998 May 29;93(5):681-4. doi: 10.1016/s0092-8674(00)81430-0.
6
Capture of an early fusion-active conformation of HIV-1 gp41.捕获HIV-1 gp41的早期融合活性构象。
Nat Struct Biol. 1998 Apr;5(4):276-9. doi: 10.1038/nsb0498-276.
7
Atomic structure of a thermostable subdomain of HIV-1 gp41.HIV-1 gp41热稳定亚结构域的原子结构。
Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12303-8. doi: 10.1073/pnas.94.23.12303.
8
The extracellular domain of immunodeficiency virus gp41 protein: expression in Escherichia coli, purification, and crystallization.免疫缺陷病毒gp41蛋白的细胞外结构域：在大肠杆菌中的表达、纯化及结晶
Protein Sci. 1997 Aug;6(8):1653-60. doi: 10.1002/pro.5560060806.
9
Atomic structure of the ectodomain from HIV-1 gp41.来自HIV-1 gp41的胞外域的原子结构。
Nature. 1997 May 22;387(6631):426-30. doi: 10.1038/387426a0.
10
Core structure of gp41 from the HIV envelope glycoprotein.来自HIV包膜糖蛋白的gp41核心结构。
Cell. 1997 Apr 18;89(2):263-73. doi: 10.1016/s0092-8674(00)80205-6.
Zotero 插件