Application of amberlite XAD-2 resin for general toxicological analysis.

The use of Amberlite XAD-2 resin for the extraction of drugs has been extended to the processing of either direct aliquots of blood and stomach contents or aqueous dialysates of the latter, as well as to that of aqueous Stas-Otto deproteinated extracts of tissue. A two-step elution provides the subsequent recovery of standard acid, neutral, basic compounds, and the more polar, water-soluble drugs and drug metabolites. Inorganics may be recovered from the eluate resulting from passage of the original solution through the resin. Extraction with columns of 1-2 g of Amberlite XAD-2 resin is shown to provide the best general approach for separations in pure form of nearly all toxic compounds from up to 500 g of tissue as well as from biological fluids and stomach contents.