一种使用琥石XAD - 2从血液中分离药物的微量方法。

A micromethod for the isolation of drugs from blood using amberlite XAD-2.

作者信息

Schlicht H J, Gelbke H P

出版信息

Z Rechtsmed. 1978 Mar 28;81(1):25-30. doi: 10.1007/BF00200596.

DOI:10.1007/BF00200596

Abstract

The extraction of drugs from small blood samples (1 ml or less) for subsequent quantitative determination is described. Isolation was carried out by adsorption of the drugs to Amberlite XAD-2 resin utilizing a batch procedure that enabled the simultaneous extraction of up to 200 samples in approx. 5 hours. A new desorption technique yielded extracts of high purity that could be used directly for gas chromatographic or radioimmunological determinations, even if hemolyzed or putrid blood was to be examined. The following 26 substances were quantitated after addition to postmortem blood speciments at concentrations of 1-10 microgram/ml: tilidine, diphenhydramine, dibenzepine, imipramine, chlorpromazine, amphetamine, pentazocine, phenacetin, methaqualone, meprobamate, parathion, diazepam, digoxin, beta-methyldigoxin, carbromal, glutethimide, amobarbital, pentobarbital, cyclobarbital, phenobarbital, diphenylhydantoin, carbutamide, tolbutamide, glycodiazin, tolazamide and chlorpropamide. Thereby recoveries of 60-100% could be achieved. The reproducibility of the procedure was satisfactory as demonstrated by coefficients of variation of 3.7-8%.

摘要

本文描述了从小血样（1毫升或更少）中提取药物用于后续定量测定的方法。采用批量法，通过将药物吸附到Amberlite XAD - 2树脂上进行分离，该方法能够在约5小时内同时提取多达200个样品。一种新的解吸技术可得到高纯度提取物，即使检测溶血或腐败血液，所得提取物也可直接用于气相色谱或放射免疫测定。在死后血液样本中添加浓度为1 - 10微克/毫升的以下26种物质进行定量分析：替利定、苯海拉明、二苯氮䓬、丙咪嗪、氯丙嗪、苯丙胺、喷他佐辛、非那西丁、甲喹酮、甲丙氨酯、对硫磷、地西泮、地高辛、β - 甲基地高辛、卡溴脲、格鲁米特、异戊巴比妥、戊巴比妥、环巴比妥、苯巴比妥、苯妥英、卡布酰胺、甲苯磺丁脲、格列本脲、甲苯磺酰胺和氯磺丙脲。回收率可达60 - 100%。该方法的重现性令人满意，变异系数为3.7 - 8%。