亚硒酸盐或硒代蛋氨酸与甲基汞在摄取和毒性方面的相互作用显示出较弱的亚硒酸盐保护作用：对培养的K-562细胞的研究

Selenite or selenomethionine interaction with methylmercury on uptake and toxicity showing a weak selenite protection: studies on cultured K-562 cells.

作者信息

Frisk P, Yaqob A, Nilsson K, Lindh U

机构信息

Department of Oncology, Radiology and Clinical Immunology, Biomedical Radiation Sciences, Uppsala University, Sweden.

出版信息

Biol Trace Elem Res. 2001 Jun;80(3):251-68. doi: 10.1385/BTER:80:3:251.

DOI:10.1385/BTER:80:3:251

PMID:11508630

Abstract

Selenium and methylmercuric chloride (MMC) interactions regarding cellular uptake and selenium protection on MMC toxicity have been studied. Human K-562 cells were pretreated or simultaneously treated with either selenite (5 or 50 microM) or selenomethionine (10 or 50 microM) together with (3.5 or 5 microM) MMC. Cells simultaneously treated with selenite or selenomethionine and 3.5 microM MMC showed a decreased mercury concentration with increased selenium dose especially seen in the selenite combinations. The simultaneous selenite and MMC 3.5 microM combinations showed growth curves with an increasing number of viable cells with increased selenite dose. All combinations with 5 microM MMC were toxic to the cells. Interactions between selenite or selenomethionine and MMC regarding cellular uptake of mercury and selenium were observed and indications of selenite protection against MMC toxicity in human K-562 cells were noticed.

摘要

研究了硒与甲基氯化汞（MMC）在细胞摄取方面的相互作用以及硒对MMC毒性的保护作用。用人K - 562细胞分别用亚硒酸盐（5或50微摩尔）或硒代蛋氨酸（10或50微摩尔）预处理或同时处理，并加入（3.5或5微摩尔）MMC。同时用亚硒酸盐或硒代蛋氨酸与3.5微摩尔MMC处理的细胞，随着硒剂量增加汞浓度降低，这在亚硒酸盐组合中尤为明显。同时用亚硒酸盐与3.5微摩尔MMC的组合显示，随着亚硒酸盐剂量增加，活细胞数量增加的生长曲线。所有与5微摩尔MMC的组合对细胞均有毒性。观察到亚硒酸盐或硒代蛋氨酸与MMC在汞和硒细胞摄取方面的相互作用，并注意到亚硒酸盐对人K - 562细胞中MMC毒性具有保护作用的迹象。