Frisk P, Yaqob A, Nilsson K, Carlsson J, Lindh U
Department of Oncology, Radiology and Clinical Immunology, Biomedical Radiation Sciences, Uppsala University, Sweden.
Biol Trace Elem Res. 2001 Sep;81(3):229-44. doi: 10.1385/BTER:81:3:229.
Selenium and mercuric chloride (MC) interactions regarding cellular uptake and selenium protection on MC toxicity have been studied. Human K-562 cells were pretreated or simultaneously treated with either selenite (5 or 50 microM) or selenomethionine (10 or 50 microM) together with MC (35 or 50 microM). Both treatments with selenite showed an increase of mercury uptake with increased selenium dose. In the pretreated or simultaneously treated selenite and 35 microM MC combinations, no inhibition of growth was seen, whereas all 50-microM MC combinations were toxic to the cells. A selenite-dependent protection was obtained for both exposure protocols when considering the cellular uptake of mercury. The cells died when the accumulation on d 4 reached more than about 0.8 x 10(-15) mol/cell of mercury, whereas they survived up to twofold more mercury uptake when exposed to selenite. Selenomethionine gave, with a few exceptions, similar effects as selenite on MC uptake and toxicity.
已对硒与氯化汞（MC）在细胞摄取方面的相互作用以及硒对MC毒性的保护作用进行了研究。人K-562细胞预先用亚硒酸盐（5或50微摩尔）或硒代蛋氨酸（10或50微摩尔）处理，或同时与MC（35或50微摩尔）一起处理。两种亚硒酸盐处理均显示随着硒剂量增加汞摄取量增加。在预先处理或同时处理的亚硒酸盐与35微摩尔MC组合中，未观察到生长抑制，而所有50微摩尔MC组合对细胞均有毒性。当考虑汞的细胞摄取时，两种暴露方案均获得了亚硒酸盐依赖性保护。当第4天的汞积累量达到约0.8×10⁻¹⁵摩尔/细胞以上时细胞死亡，而当暴露于亚硒酸盐时，细胞在汞摄取量增加两倍的情况下仍能存活。除了少数例外，硒代蛋氨酸对MC摄取和毒性的影响与亚硒酸盐相似。
