Raven J L, Robson M B
J Clin Pathol. 1975 Jul;28(7):531-9. doi: 10.1136/jcp.28.7.531.
The protein precipitates discarded during the extraction process of the Lactobacillus leichmannii vitamin B12 assay have been shown to contain significant amounts of vitamin B12. This loss of vitamin B12 provide a satisfactory explanation for many of the descrepancies between the serum vitamin B12 values obtained by the L. leichmannii method and the radio-isotopic method of Raven et al (1969). It is possible to produce lower results by the method of Raven et al (1969)by incorporating into that method the L. leichmannii extraction process; it is also possible to produce higher results by the L. leichmannii method using a papain extraction process.
在赖氏乳杆菌维生素B12测定的提取过程中被丢弃的蛋白质沉淀物已被证明含有大量的维生素B12。维生素B12的这种损失为赖氏乳杆菌法与Raven等人（1969年）的放射性同位素法所获得的血清维生素B12值之间的许多差异提供了一个令人满意的解释。通过将赖氏乳杆菌提取过程纳入Raven等人（1969年）的方法中，有可能得到更低的结果；使用木瓜蛋白酶提取过程的赖氏乳杆菌法也有可能得到更高的结果。
