使用一种用于血清中维生素B12放射性同位素测定的商用试剂盒的经验：法德巴斯B12检测
Experience with a commercial kit for the radioisotopic assay of vitamin B12 in serum: the Phadebas B12 test.
作者信息
Raven J L, Robson M B
出版信息
J Clin Pathol. 1974 Jan;27(1):59-65. doi: 10.1136/jcp.27.1.59.
Abstract
The first commercial kit for the radioisotopic assay of vitamin B(12) in serum-the Phadebas B(12) Test produced higher values than the radioisotopic method of Raven, Robson, Walker, and Barkham (1969) and the Lactobacillus leichmannii microbiological assay. Its normal range was 300-1100 pg/ml and its reproducibility was similar to that of the other radioisotopic method. It should be possible to lower the results obtained by the Phadebas method by modifying its standard curve and to reduce the time taken for the assay by shortening its incubation period.
摘要
首个用于血清中维生素B12放射性同位素测定的商业试剂盒——法德巴斯B12检测试剂盒，其检测值高于雷文、罗布森、沃克和巴克姆（1969年）的放射性同位素法以及赖希曼氏乳酸杆菌微生物检测法。其正常范围为300 - 1100 pg/ml，其重现性与另一种放射性同位素法相似。通过修改其标准曲线应该可以降低法德巴斯法获得的结果，并通过缩短孵育期来减少检测所需时间。
相似文献
1
Experience with a commercial kit for the radioisotopic assay of vitamin B12 in serum: the Phadebas B12 test.使用一种用于血清中维生素B12放射性同位素测定的商用试剂盒的经验：法德巴斯B12检测
J Clin Pathol. 1974 Jan;27(1):59-65. doi: 10.1136/jcp.27.1.59.
2
Results with radioisotopic assay of serum B12 using serum binding agent.采用血清结合剂对血清维生素B12进行放射性同位素测定的结果。
J Clin Pathol. 1967 Sep;20(5):683-6. doi: 10.1136/jcp.20.5.683.
3
The serum vitamin B12 level: its assay and significance.血清维生素B12水平：其测定方法及意义。
Clin Haematol. 1976 Oct;5(3):521-46.
4
Comparison of three methods for measuring vitamin B 12 in serum: radioisotopic, euglena gracilis and Lactobacillus leichmannii.血清中维生素B₁₂三种检测方法的比较：放射性同位素法、纤细裸藻法和赖氏乳杆菌法。
Br J Haematol. 1972 Jan;22(1):21-31. doi: 10.1111/j.1365-2141.1972.tb08783.x.
5
An evaluation of commercial radioisotope methods for the determination of folate and vitamin B12.用于测定叶酸和维生素B12的商用放射性同位素方法的评估。
J Clin Pathol. 1980 Mar;33(3):234-42. doi: 10.1136/jcp.33.3.234.
6
The usefulness of radioisotopic dilution assay in a population with high serum vitamin B12 concentration.放射性同位素稀释法在血清维生素B12浓度较高人群中的实用性。
Acta Vitaminol Enzymol. 1981;3(1):27-30.
7
The use of chicken serum for measurement of serum vitamin B12 concentration by radioisotope dilution: discription of method and comparison with microbiological assay results.用鸡血清通过放射性同位素稀释法测定血清维生素B12浓度：方法描述及与微生物学测定结果的比较。
Br J Haematol. 1974 Jul;27(3):507-26. doi: 10.1111/j.1365-2141.1974.tb06816.x.
8
Extraction of serum vitamin B12 for radio-isotopic and Lactobacillus leichmannii assay.用于放射性同位素和赖氏乳杆菌检测的血清维生素B12提取。
J Clin Pathol. 1975 Jul;28(7):531-9. doi: 10.1136/jcp.28.7.531.
9
Underestimation of vitamin B 12 in serum by a microbiological method(Lactobacillus leichmannii): comparison with a radioisotopic method.用微生物学方法（赖氏乳杆菌）测定血清中维生素B12含量的结果偏低：与放射性同位素法的比较
J Clin Pathol. 1969 Nov;22(6):741. doi: 10.1136/jcp.22.6.741-b.
10
A comparison of radioisotopic and bioassay methods of measurement of serum vitamin B 12.血清维生素B₁₂测量的放射性同位素法与生物测定法的比较。
Nutr Rev. 1972 Sep;30(9):209-11. doi: 10.1111/j.1753-4887.1972.tb04045.x.
引用本文的文献
1
An evaluation of commercial radioisotope methods for the determination of folate and vitamin B12.用于测定叶酸和维生素B12的商用放射性同位素方法的评估。
J Clin Pathol. 1980 Mar;33(3):234-42. doi: 10.1136/jcp.33.3.234.
2
Comparison of serum vitamin B12 estimation by saturation analysis with intrinsic factor and with R-protein as binding agents.采用内因子和R蛋白作为结合剂，通过饱和分析法对血清维生素B12进行测定的比较。
J Clin Pathol. 1981 Apr;34(4):357-60. doi: 10.1136/jcp.34.4.357.
3
Interlaboratory comparison of serum vitamin B12 assay.血清维生素B12检测的实验室间比对
J Clin Pathol. 1980 Mar;33(3):243-8. doi: 10.1136/jcp.33.3.243.
4
Megaloblastic anaemia, cobalamin, and folate.巨幼细胞贫血、钴胺素和叶酸。
J Clin Pathol. 1987 Sep;40(9):978-84. doi: 10.1136/jcp.40.9.978.
5
Extraction of serum vitamin B12 for radio-isotopic and Lactobacillus leichmannii assay.用于放射性同位素和赖氏乳杆菌检测的血清维生素B12提取。
J Clin Pathol. 1975 Jul;28(7):531-9. doi: 10.1136/jcp.28.7.531.
6
The influence of serum folate on urinary excretion of vitamin B12.血清叶酸对维生素B12尿排泄的影响。
Eur J Nucl Med. 1978 Jul 1;3(3):175-8. doi: 10.1007/BF00256639.
本文引用的文献
1
Observations on the estimation of serum vitamin B12 using Lactobacillus leichmannii.使用赖氏乳杆菌对血清维生素B12进行测定的观察
Clin Sci. 1962 Feb;22:101-11.
2
3
An improved method for the rapid estimation of vitamin B12 in serum.一种改进的血清中维生素B12快速估测方法。
Clin Sci. 1955 Nov;14(4):661-7.
4
The determination of vitamin B12 activity in human serum.人血清中维生素B12活性的测定
J Biol Chem. 1952 Nov;199(1):433-42.
5
Addisonian pernicious anaemia and intrinsic factor antibodies in thyroid disorders.甲状腺疾病中的艾迪生病性恶性贫血与内因子抗体
Q J Med. 1966 Jul;35(139):421-31.
6
Improved method for measuring vitamin B12 in serum using intrinsic factor, 57CoB12, and coated charcoal.使用内因子、57钴维生素B12和包被活性炭测定血清中维生素B12的改进方法
J Clin Pathol. 1969 Mar;22(2):205-11. doi: 10.1136/jcp.22.2.205.
7
A radiosorbent technique for the assay of serum vitamin B12.一种用于测定血清维生素B12的放射吸附技术。
Scand J Clin Lab Invest. 1971 Apr;27(2):151-9. doi: 10.3109/00365517109080202.
8
9
Comparison of two radioassay methods for vitamin B 12 .两种维生素B₁₂放射测定方法的比较。
Clin Chem. 1972 Jul;18(7):740-1.
Zotero 插件