使用一种用于血清中维生素B12放射性同位素测定的商用试剂盒的经验：法德巴斯B12检测
Experience with a commercial kit for the radioisotopic assay of vitamin B12 in serum: the Phadebas B12 test.
作者信息
Raven J L, Robson M B
出版信息
J Clin Pathol. 1974 Jan;27(1):59-65. doi: 10.1136/jcp.27.1.59.
Abstract
The first commercial kit for the radioisotopic assay of vitamin B(12) in serum-the Phadebas B(12) Test produced higher values than the radioisotopic method of Raven, Robson, Walker, and Barkham (1969) and the Lactobacillus leichmannii microbiological assay. Its normal range was 300-1100 pg/ml and its reproducibility was similar to that of the other radioisotopic method. It should be possible to lower the results obtained by the Phadebas method by modifying its standard curve and to reduce the time taken for the assay by shortening its incubation period.
摘要
首个用于血清中维生素B12放射性同位素测定的商业试剂盒——法德巴斯B12检测试剂盒，其检测值高于雷文、罗布森、沃克和巴克姆（1969年）的放射性同位素法以及赖希曼氏乳酸杆菌微生物检测法。其正常范围为300 - 1100 pg/ml，其重现性与另一种放射性同位素法相似。通过修改其标准曲线应该可以降低法德巴斯法获得的结果，并通过缩短孵育期来减少检测所需时间。
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