Hur J Y, Soh Y, Kim B H, Suk K, Sohn N W, Kim H C, Kwon H C, Lee K R, Kim S Y
Graduate School of East-West Medical Science, Kyung Hee University, Seoul, Korea.
Biol Pharm Bull. 2001 Aug;24(8):921-4. doi: 10.1248/bpb.24.921.
Aster scaber T. (Asteraceae) has been used to treat bruises, snakebite, headache, and dizziness in traditional Chinese medicine. In the present study, the neuroprotective effect of four quinic acid derivatives from A. scaber on amyloid Abeta-induced PC12 cell toxicity was investigated. When cells were treated with quinic acid derivatives prior to Abeta, cell toxicity was significantly diminished. Among quinic acid derivatives, (-)4,5-dicaffeoyl quinic acid (1) gave the highest protection against Abeta-induced cell toxicity. In addition, the neurotrophic effects of compounds were evaluated by microscopically monitoring their potency to induce neurite outgrowth in PC12 cells. Four quinic acid derivatives from A. scaber promoted neurite outgrowth in PC12 cells. Interestingly, a novel quinic acid, (-)3,5-dicaffeoyl-muco-quinic acid (2) was more effective than the other compounds in promoting neurite outgrowth. Unlike nerve growth factor, the withdrawal of quinic acids did not result in any significant decrease in cell viability. The results suggest that quinic acid derivatives from A. scaber might potentially be used as a therapeutic agent in Alzheimer disease.
紫菀（菊科）在传统中药中被用于治疗瘀伤、蛇咬伤、头痛和头晕。在本研究中，研究了紫菀中四种奎宁酸衍生物对淀粉样β蛋白诱导的PC12细胞毒性的神经保护作用。当细胞在β淀粉样蛋白处理之前先用奎宁酸衍生物处理时，细胞毒性显著降低。在奎宁酸衍生物中，（-）4,5-二咖啡酰奎宁酸（1）对β淀粉样蛋白诱导的细胞毒性具有最高的保护作用。此外，通过显微镜监测化合物诱导PC12细胞神经突生长的能力来评估其神经营养作用。紫菀中的四种奎宁酸衍生物促进了PC12细胞的神经突生长。有趣的是，一种新型奎宁酸，（-）3,5-二咖啡酰-粘质奎宁酸（2）在促进神经突生长方面比其他化合物更有效。与神经生长因子不同，去除奎宁酸不会导致细胞活力有任何显著下降。结果表明，紫菀中的奎宁酸衍生物可能有潜力用作治疗阿尔茨海默病的药物。
