Chung Ill-Min, Kim Min-Young, Park Won-Hwan, Moon Hyung-In
Department of Applied Life science, Kon Kuk University, Seoul.
J Enzyme Inhib Med Chem. 2009 Feb;24(1):188-91. doi: 10.1080/14756360802051230.
The leaves of Saussurea triangulata (Compositae) have been eaten with rice as a wrapping vegetable for preventing neuro-aging. However, the components responsible for the neuroprotective effects of S. triangulata still remain unidentified. In the process of investigating the neuroprotective activity of S. triangulata, we found that a methanol extract of S. triangulata exhibited significant protection against glutamate-induced toxicity in primary cultured rat cortical cells. Three quinic acid derivatives were isolated from the n-BuOH fraction of S. triangulata. Among these three quinic acid derivatives, methyl 5-caffeoylquinic acid (3) exhibited significant neuroprotective activities against glutamate-induced toxicity exhibiting cell viability of about 50%, at concentrations ranging from 0.1 microM to 10 microM. Therefore, the neuroprotective effect of S. triangulata might be due to the inhibition of glutamate-induced toxicity by the quinic acid derivatives from S. triangulata.
三角叶风毛菊（菊科）的叶子与米饭一起食用，作为一种包裹蔬菜，用于预防神经衰老。然而，三角叶风毛菊具有神经保护作用的成分仍未明确。在研究三角叶风毛菊神经保护活性的过程中，我们发现三角叶风毛菊的甲醇提取物对原代培养的大鼠皮质细胞中谷氨酸诱导的毒性具有显著的保护作用。从三角叶风毛菊的正丁醇部位分离出三种奎宁酸衍生物。在这三种奎宁酸衍生物中，5-咖啡酰奎宁酸甲酯（3）在0.1微摩尔至10微摩尔的浓度范围内，对谷氨酸诱导的毒性表现出显著的神经保护活性，细胞活力约为50%。因此，三角叶风毛菊的神经保护作用可能是由于其含有的奎宁酸衍生物抑制了谷氨酸诱导的毒性。
