Schroeder W A, Evans L, Grussing L, Abraham E C, Huisman T H, Lam H, Shelton J B
Am J Hematol. 1976;1(3):331-8. doi: 10.1002/ajh.2830010308.
The improved microchromatographic procedure for the detection of Hb-S and/or Hb-C in cord blood at birth (Schroeder et al.: J. Lab Clin Med 86:528-532, 1975) as well as a modification thereof may also be used for the quantitative determination of Hb-F in the presence of Hb-S and/or Hb-C. However, Hb-A interferes and must be absent. The methods use 0.5 X 6 cm columns of CM-cellulose with Tirs or Bis-tris developers and require 2-4 hr to complete. At low percentages of Hb-F, the sharper zone of the Tris method is more easily visible than that of the Bis-tris method, but the latter is a somewhat more rapid procedure. About 300 cases with Hb-S and/or Hb-C have been examined by the microchromatographic procedure. Most of these results (Fmicro) have been compared with data from the determination of Hb-F by one or more of the following methods: alkali denaturation (FAD), conventional DEAE-Sephadex chromatography (FDES), or isoleucine analyses of zones from DEAE-Sephadex chromatography (FIle). The accuracy and precision of the microchromatographic method is estimated to be 5-10%. The microchromatographic methods require much less time than conventional chromatography but more time than alkali denaturation procedures. Compared to the latter, the new methods use whole blood and less blood and permit the physical separation of Hb-F.
用于出生时检测脐血中血红蛋白 S 和/或血红蛋白 C 的改良微量色谱法（施罗德等人：《临床检验杂志》86:528 - 532，1975）及其一种改进方法也可用于在存在血红蛋白 S 和/或血红蛋白 C 的情况下定量测定血红蛋白 F。然而，血红蛋白 A 会产生干扰，必须不存在。这些方法使用 0.5×6 厘米的羧甲基纤维素柱，以三羟甲基氨基甲烷（Tirs）或双三羟甲基氨基甲烷（Bis - tris）作为展开剂，需要 2 - 4 小时完成。在血红蛋白 F 含量较低时，三羟甲基氨基甲烷法的较清晰区带比双三羟甲基氨基甲烷法的更易观察到，但后者的操作过程稍快一些。已经通过微量色谱法检查了约 300 例血红蛋白 S 和/或血红蛋白 C 的病例。这些结果中的大多数（Fmicro）已与通过以下一种或多种方法测定血红蛋白 F 得到的数据进行了比较：碱变性法（FAD）、传统的二乙氨基乙基 - 葡聚糖凝胶色谱法（FDES）或对二乙氨基乙基 - 葡聚糖凝胶色谱区带进行异亮氨酸分析（FIle）。微量色谱法的准确度和精密度估计为 5 - 10%。微量色谱法所需时间比传统色谱法少得多，但比碱变性法多。与后者相比，新方法使用全血且血量较少，并能对血红蛋白 F 进行物理分离。
